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Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent

Bacterial cellulose (BC) produced by Gluconoacetobacter hansenii is a suitable polymeric fiber network for wound-dressing purposes, but its lack of antibacterial properties limits it from healing bacterial wounds. We developed hydrogels by impregnating fungal-derived carboxymethyl chitosan to BC fib...

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Detalles Bibliográficos
Autores principales: Suneetha, Maduru, Won, So-Yeon, Zo, Sun Mi, Han, Sung Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048145/
https://www.ncbi.nlm.nih.gov/pubmed/36975633
http://dx.doi.org/10.3390/gels9030184
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author Suneetha, Maduru
Won, So-Yeon
Zo, Sun Mi
Han, Sung Soo
author_facet Suneetha, Maduru
Won, So-Yeon
Zo, Sun Mi
Han, Sung Soo
author_sort Suneetha, Maduru
collection PubMed
description Bacterial cellulose (BC) produced by Gluconoacetobacter hansenii is a suitable polymeric fiber network for wound-dressing purposes, but its lack of antibacterial properties limits it from healing bacterial wounds. We developed hydrogels by impregnating fungal-derived carboxymethyl chitosan to BC fiber networks using a simple solution immersion method. The CMCS–BC hydrogels were characterized using various characterization techniques such as XRD, FTIR, water contact angle measurements, TGA, and SEM to know the physiochemical properties. The results show that the impregnation of CMCS into BC fiber networks greatly influences BC’s improving hydrophilic nature, which is crucial for wound healing applications. Furthermore, the CMCS–BC hydrogels were studied for biocompatibility analysis with skin fibroblast cells. The results revealed that by increasing the CMCS content in the BC, biocompatibility, cell attachment, and spreading capacity also increase. The antibacterial activity of CMCS–BC hydrogels is shown using the CFU method against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). As a result, the CMCS–BC hydrogels exhibit more suitable antibacterial properties than those without BC due to the CMCS having amino groups that enhance antibacterial properties. Therefore, CMCS–BC hydrogels can be considered suitable for antibacterial wound dressing applications.
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spelling pubmed-100481452023-03-29 Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent Suneetha, Maduru Won, So-Yeon Zo, Sun Mi Han, Sung Soo Gels Article Bacterial cellulose (BC) produced by Gluconoacetobacter hansenii is a suitable polymeric fiber network for wound-dressing purposes, but its lack of antibacterial properties limits it from healing bacterial wounds. We developed hydrogels by impregnating fungal-derived carboxymethyl chitosan to BC fiber networks using a simple solution immersion method. The CMCS–BC hydrogels were characterized using various characterization techniques such as XRD, FTIR, water contact angle measurements, TGA, and SEM to know the physiochemical properties. The results show that the impregnation of CMCS into BC fiber networks greatly influences BC’s improving hydrophilic nature, which is crucial for wound healing applications. Furthermore, the CMCS–BC hydrogels were studied for biocompatibility analysis with skin fibroblast cells. The results revealed that by increasing the CMCS content in the BC, biocompatibility, cell attachment, and spreading capacity also increase. The antibacterial activity of CMCS–BC hydrogels is shown using the CFU method against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). As a result, the CMCS–BC hydrogels exhibit more suitable antibacterial properties than those without BC due to the CMCS having amino groups that enhance antibacterial properties. Therefore, CMCS–BC hydrogels can be considered suitable for antibacterial wound dressing applications. MDPI 2023-02-27 /pmc/articles/PMC10048145/ /pubmed/36975633 http://dx.doi.org/10.3390/gels9030184 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suneetha, Maduru
Won, So-Yeon
Zo, Sun Mi
Han, Sung Soo
Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title_full Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title_fullStr Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title_full_unstemmed Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title_short Fungal Carboxymethyl Chitosan-Impregnated Bacterial Cellulose Hydrogel as Wound-Dressing Agent
title_sort fungal carboxymethyl chitosan-impregnated bacterial cellulose hydrogel as wound-dressing agent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048145/
https://www.ncbi.nlm.nih.gov/pubmed/36975633
http://dx.doi.org/10.3390/gels9030184
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