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Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections
Diabetic foot ulcers (DFU) are among the most common complications in diabetic patients and affect 6.8% of people worldwide. Challenges in the management of this disease are decreased blood diffusion, sclerotic tissues, infection, and antibiotic resistance. Hydrogels are now being used as a new trea...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048173/ https://www.ncbi.nlm.nih.gov/pubmed/36975711 http://dx.doi.org/10.3390/gels9030262 |
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author | Chijcheapaza-Flores, Henry Tabary, Nicolas Chai, Feng Maton, Mickaël Staelens, Jean-Noel Cazaux, Frédéric Neut, Christel Martel, Bernard Blanchemain, Nicolas Garcia-Fernandez, Maria José |
author_facet | Chijcheapaza-Flores, Henry Tabary, Nicolas Chai, Feng Maton, Mickaël Staelens, Jean-Noel Cazaux, Frédéric Neut, Christel Martel, Bernard Blanchemain, Nicolas Garcia-Fernandez, Maria José |
author_sort | Chijcheapaza-Flores, Henry |
collection | PubMed |
description | Diabetic foot ulcers (DFU) are among the most common complications in diabetic patients and affect 6.8% of people worldwide. Challenges in the management of this disease are decreased blood diffusion, sclerotic tissues, infection, and antibiotic resistance. Hydrogels are now being used as a new treatment option since they can be used for drug delivery and to improve wound healing. This project aims to combine the properties of hydrogels based on chitosan (CHT) and the polymer of β cyclodextrin (PCD) for local delivery of cinnamaldehyde (CN) in diabetic foot ulcers. This work consisted of the development and characterisation of the hydrogel, the evaluation of the CN release kinetics and cell viability (on a MC3T3 pre-osteoblast cell line), and the evaluation of the antimicrobial and antibiofilm activity (S. aureus and P. aeruginosa). The results demonstrated the successful development of a cytocompatible (ISO 10993-5) injectable hydrogel with antibacterial (99.99% bacterial reduction) and antibiofilm activity. Furthermore, a partial active molecule release and an increase in hydrogel elasticity were observed in the presence of CN. This leads us to hypothesise that a reaction between CHT and CN (a Schiff base) can occur and that CN could act as a physical crosslinker, thus improving the viscoelastic properties of the hydrogel and limiting CN release. |
format | Online Article Text |
id | pubmed-10048173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100481732023-03-29 Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections Chijcheapaza-Flores, Henry Tabary, Nicolas Chai, Feng Maton, Mickaël Staelens, Jean-Noel Cazaux, Frédéric Neut, Christel Martel, Bernard Blanchemain, Nicolas Garcia-Fernandez, Maria José Gels Article Diabetic foot ulcers (DFU) are among the most common complications in diabetic patients and affect 6.8% of people worldwide. Challenges in the management of this disease are decreased blood diffusion, sclerotic tissues, infection, and antibiotic resistance. Hydrogels are now being used as a new treatment option since they can be used for drug delivery and to improve wound healing. This project aims to combine the properties of hydrogels based on chitosan (CHT) and the polymer of β cyclodextrin (PCD) for local delivery of cinnamaldehyde (CN) in diabetic foot ulcers. This work consisted of the development and characterisation of the hydrogel, the evaluation of the CN release kinetics and cell viability (on a MC3T3 pre-osteoblast cell line), and the evaluation of the antimicrobial and antibiofilm activity (S. aureus and P. aeruginosa). The results demonstrated the successful development of a cytocompatible (ISO 10993-5) injectable hydrogel with antibacterial (99.99% bacterial reduction) and antibiofilm activity. Furthermore, a partial active molecule release and an increase in hydrogel elasticity were observed in the presence of CN. This leads us to hypothesise that a reaction between CHT and CN (a Schiff base) can occur and that CN could act as a physical crosslinker, thus improving the viscoelastic properties of the hydrogel and limiting CN release. MDPI 2023-03-22 /pmc/articles/PMC10048173/ /pubmed/36975711 http://dx.doi.org/10.3390/gels9030262 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chijcheapaza-Flores, Henry Tabary, Nicolas Chai, Feng Maton, Mickaël Staelens, Jean-Noel Cazaux, Frédéric Neut, Christel Martel, Bernard Blanchemain, Nicolas Garcia-Fernandez, Maria José Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title | Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title_full | Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title_fullStr | Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title_full_unstemmed | Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title_short | Injectable Chitosan-Based Hydrogels for Trans-Cinnamaldehyde Delivery in the Treatment of Diabetic Foot Ulcer Infections |
title_sort | injectable chitosan-based hydrogels for trans-cinnamaldehyde delivery in the treatment of diabetic foot ulcer infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048173/ https://www.ncbi.nlm.nih.gov/pubmed/36975711 http://dx.doi.org/10.3390/gels9030262 |
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