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Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys
Obesity is a metabolic disorder resulting from behavioral, environmental and heritable causes, and can have a negative impact on male reproduction. There have been few experiments in mice, rats, and rabbits on the effects of obesity on reproduction, which has inhibited the development of better trea...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048326/ https://www.ncbi.nlm.nih.gov/pubmed/36980830 http://dx.doi.org/10.3390/genes14030557 |
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author | Zhang, Yanru Qi, Jia Zhao, Juan Li, Miaojing Zhang, Yulin Hu, Huizhong Wei, Liangliang Zhou, Kai Qin, Hongyu Qu, Pengxiang Cao, Wenbin Liu, Enqi |
author_facet | Zhang, Yanru Qi, Jia Zhao, Juan Li, Miaojing Zhang, Yulin Hu, Huizhong Wei, Liangliang Zhou, Kai Qin, Hongyu Qu, Pengxiang Cao, Wenbin Liu, Enqi |
author_sort | Zhang, Yanru |
collection | PubMed |
description | Obesity is a metabolic disorder resulting from behavioral, environmental and heritable causes, and can have a negative impact on male reproduction. There have been few experiments in mice, rats, and rabbits on the effects of obesity on reproduction, which has inhibited the development of better treatments for male subfertility caused by obesity. Nonhuman primates are most similar to human beings in anatomy, physiology, metabolism, and biochemistry and are appropriate subjects for obesity studies. In this investigation, we conducted a transcriptome analysis of the testes of cynomolgus monkeys on high-fat, high-fructose, and cholesterol-rich diets to determine the effect of obesity on gene expression in testes. The results showed that the testes of obese monkeys had abnormal morphology, and their testes transcriptome was significantly different from that of non-obese animals. We identified 507 differentially abundant genes (adjusted p value < 0.01, log2 [FC] > 2) including 163 up-regulated and 344 down-regulated genes. Among the differentially abundant genes were ten regulatory genes, including IRF1, IRF6, HERC5, HERC6, IFIH1, IFIT2, IFIT5, IFI35, RSAD2, and UBQLNL. Gene ontology (GO) and KEGG pathway analysis was conducted, and we found that processes and pathways associated with the blood testes barrier (BTB), immunity, inflammation, and DNA methylation in gametes were preferentially enriched. We also found abnormal expression of genes related to infertility (TDRD5, CLCN2, MORC1, RFX8, SOHLH1, IL2RB, MCIDAS, ZPBP, NFIA, PTPN11, TSC22D3, MAPK6, PLCB1, DCUN1D1, LPIN1, and GATM) and down-regulation of testosterone in monkeys with dietetic obesity. This work not only provides an important reference for research and treatment on male infertility caused by obesity, but also valuable insights into the effects of diet on gene expression in testes. |
format | Online Article Text |
id | pubmed-10048326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100483262023-03-29 Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys Zhang, Yanru Qi, Jia Zhao, Juan Li, Miaojing Zhang, Yulin Hu, Huizhong Wei, Liangliang Zhou, Kai Qin, Hongyu Qu, Pengxiang Cao, Wenbin Liu, Enqi Genes (Basel) Article Obesity is a metabolic disorder resulting from behavioral, environmental and heritable causes, and can have a negative impact on male reproduction. There have been few experiments in mice, rats, and rabbits on the effects of obesity on reproduction, which has inhibited the development of better treatments for male subfertility caused by obesity. Nonhuman primates are most similar to human beings in anatomy, physiology, metabolism, and biochemistry and are appropriate subjects for obesity studies. In this investigation, we conducted a transcriptome analysis of the testes of cynomolgus monkeys on high-fat, high-fructose, and cholesterol-rich diets to determine the effect of obesity on gene expression in testes. The results showed that the testes of obese monkeys had abnormal morphology, and their testes transcriptome was significantly different from that of non-obese animals. We identified 507 differentially abundant genes (adjusted p value < 0.01, log2 [FC] > 2) including 163 up-regulated and 344 down-regulated genes. Among the differentially abundant genes were ten regulatory genes, including IRF1, IRF6, HERC5, HERC6, IFIH1, IFIT2, IFIT5, IFI35, RSAD2, and UBQLNL. Gene ontology (GO) and KEGG pathway analysis was conducted, and we found that processes and pathways associated with the blood testes barrier (BTB), immunity, inflammation, and DNA methylation in gametes were preferentially enriched. We also found abnormal expression of genes related to infertility (TDRD5, CLCN2, MORC1, RFX8, SOHLH1, IL2RB, MCIDAS, ZPBP, NFIA, PTPN11, TSC22D3, MAPK6, PLCB1, DCUN1D1, LPIN1, and GATM) and down-regulation of testosterone in monkeys with dietetic obesity. This work not only provides an important reference for research and treatment on male infertility caused by obesity, but also valuable insights into the effects of diet on gene expression in testes. MDPI 2023-02-23 /pmc/articles/PMC10048326/ /pubmed/36980830 http://dx.doi.org/10.3390/genes14030557 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Yanru Qi, Jia Zhao, Juan Li, Miaojing Zhang, Yulin Hu, Huizhong Wei, Liangliang Zhou, Kai Qin, Hongyu Qu, Pengxiang Cao, Wenbin Liu, Enqi Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title | Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title_full | Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title_fullStr | Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title_full_unstemmed | Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title_short | Effect of Dietetic Obesity on Testicular Transcriptome in Cynomolgus Monkeys |
title_sort | effect of dietetic obesity on testicular transcriptome in cynomolgus monkeys |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048326/ https://www.ncbi.nlm.nih.gov/pubmed/36980830 http://dx.doi.org/10.3390/genes14030557 |
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