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Development of Improved DNA Collection and Extraction Methods for Handled Documents
Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048427/ https://www.ncbi.nlm.nih.gov/pubmed/36981031 http://dx.doi.org/10.3390/genes14030761 |
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author | Morgan, Ashley G. Prinz, Mechthild |
author_facet | Morgan, Ashley G. Prinz, Mechthild |
author_sort | Morgan, Ashley G. |
collection | PubMed |
description | Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span. |
format | Online Article Text |
id | pubmed-10048427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100484272023-03-29 Development of Improved DNA Collection and Extraction Methods for Handled Documents Morgan, Ashley G. Prinz, Mechthild Genes (Basel) Article Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span. MDPI 2023-03-21 /pmc/articles/PMC10048427/ /pubmed/36981031 http://dx.doi.org/10.3390/genes14030761 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morgan, Ashley G. Prinz, Mechthild Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title | Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title_full | Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title_fullStr | Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title_full_unstemmed | Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title_short | Development of Improved DNA Collection and Extraction Methods for Handled Documents |
title_sort | development of improved dna collection and extraction methods for handled documents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048427/ https://www.ncbi.nlm.nih.gov/pubmed/36981031 http://dx.doi.org/10.3390/genes14030761 |
work_keys_str_mv | AT morganashleyg developmentofimproveddnacollectionandextractionmethodsforhandleddocuments AT prinzmechthild developmentofimproveddnacollectionandextractionmethodsforhandleddocuments |