Enhancing the Hydrolysis and Acyl Transfer Activity of Carboxylesterase DLFae4 by a Combinational Mutagenesis and In-Silico Method

In the present study, a feruloyl esterase DLFae4 identified in our previous research was modified by error-prone PCR and site-directed saturation mutation to enhance the catalytic efficiency and acyltransferase activity further. Five mutants with 6.9–118.9% enhanced catalytic activity toward methyl...

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Detalles Bibliográficos
Autores principales: Li, Longxiang, Ding, Liping, Shao, Yuting, Sun, Shengwei, Wang, Mengxi, Xiang, Jiahui, Zhou, Jingjie, Wu, Guojun, Song, Zhe, Xin, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048530/
https://www.ncbi.nlm.nih.gov/pubmed/36981096
http://dx.doi.org/10.3390/foods12061169

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