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Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules
The first element legislated adopting chemical speciation was chromium (Cr) for differentiation between the highly toxic Cr(VI) from the micronutrient Cr(III). Therefore, this work aimed to develop a new analytical method through the coupling of High-Performance Liquid Chromatography with Diode-Arra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049150/ https://www.ncbi.nlm.nih.gov/pubmed/36981823 http://dx.doi.org/10.3390/ijerph20064912 |
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author | Procópio, Vitória Aparecida Pereira, Rodrigo Mendes Lange, Camila Neves Freire, Bruna Moreira Batista, Bruno Lemos |
author_facet | Procópio, Vitória Aparecida Pereira, Rodrigo Mendes Lange, Camila Neves Freire, Bruna Moreira Batista, Bruno Lemos |
author_sort | Procópio, Vitória Aparecida |
collection | PubMed |
description | The first element legislated adopting chemical speciation was chromium (Cr) for differentiation between the highly toxic Cr(VI) from the micronutrient Cr(III). Therefore, this work aimed to develop a new analytical method through the coupling of High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) with inductively coupled plasma mass spectrometry (ICP-MS) to obtain molecular and elemental information simultaneously from a single sample injection. In the first step, a low-cost flow split made of acrylic was developed aiming at optimally directing the sample to the detectors, enabling the HPLC-DAD/ICP-MS coupling. After the extraction of Certified Reference Materials (CRM of natural water NIST1640a and sugar cane leaf agro FC_012017), the recoveries determined by ICP-MS were 99.7% and 85.4%, respectively. Then, the method of HPLC-DAD/ICP-MS was applied for real samples of the CRMs. The presence of possible biomolecules associated with Cr(III) and Cr(VI) species was evaluated, with the simultaneous response detection of molecular (DAD) and elementary (ICP-MS) detectors. Potential biomolecules were observed during the monitoring of Cr(VI) and Cr(III) in sugar cane leaves, water samples and a supplement of Cr picolinate. Finally, the article also discusses the potential of the technique applied to biomolecules containing other associated elements and the need of more bioanalytical methods to understand the presence of trace elements in biomolecules. |
format | Online Article Text |
id | pubmed-10049150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100491502023-03-29 Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules Procópio, Vitória Aparecida Pereira, Rodrigo Mendes Lange, Camila Neves Freire, Bruna Moreira Batista, Bruno Lemos Int J Environ Res Public Health Article The first element legislated adopting chemical speciation was chromium (Cr) for differentiation between the highly toxic Cr(VI) from the micronutrient Cr(III). Therefore, this work aimed to develop a new analytical method through the coupling of High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) with inductively coupled plasma mass spectrometry (ICP-MS) to obtain molecular and elemental information simultaneously from a single sample injection. In the first step, a low-cost flow split made of acrylic was developed aiming at optimally directing the sample to the detectors, enabling the HPLC-DAD/ICP-MS coupling. After the extraction of Certified Reference Materials (CRM of natural water NIST1640a and sugar cane leaf agro FC_012017), the recoveries determined by ICP-MS were 99.7% and 85.4%, respectively. Then, the method of HPLC-DAD/ICP-MS was applied for real samples of the CRMs. The presence of possible biomolecules associated with Cr(III) and Cr(VI) species was evaluated, with the simultaneous response detection of molecular (DAD) and elementary (ICP-MS) detectors. Potential biomolecules were observed during the monitoring of Cr(VI) and Cr(III) in sugar cane leaves, water samples and a supplement of Cr picolinate. Finally, the article also discusses the potential of the technique applied to biomolecules containing other associated elements and the need of more bioanalytical methods to understand the presence of trace elements in biomolecules. MDPI 2023-03-10 /pmc/articles/PMC10049150/ /pubmed/36981823 http://dx.doi.org/10.3390/ijerph20064912 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Procópio, Vitória Aparecida Pereira, Rodrigo Mendes Lange, Camila Neves Freire, Bruna Moreira Batista, Bruno Lemos Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title | Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title_full | Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title_fullStr | Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title_full_unstemmed | Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title_short | Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules |
title_sort | chromium speciation by hplc-dad/icp-ms: simultaneous hyphenation of analytical techniques for studies of biomolecules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049150/ https://www.ncbi.nlm.nih.gov/pubmed/36981823 http://dx.doi.org/10.3390/ijerph20064912 |
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