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Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study

The risk of toxicity attributable to radioiodine therapy (RIT) remains a subject of ongoing research, with a whole-body dose of 2 Gy proposed as a safe limit. This article evaluates the RIT-induced cytogenetic damage in two rare differentiated thyroid cancer (DTC) cases, including the first follow-u...

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Autores principales: Khvostunov, Igor K., Nasonova, Elena, Krylov, Valeriy, Rodichev, Andrei, Kochetova, Tatiana, Shepel, Natalia, Korovchuk, Olga, Kutsalo, Polina, Shegai, Petr, Kaprin, Andrei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049272/
https://www.ncbi.nlm.nih.gov/pubmed/36982202
http://dx.doi.org/10.3390/ijms24065128
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author Khvostunov, Igor K.
Nasonova, Elena
Krylov, Valeriy
Rodichev, Andrei
Kochetova, Tatiana
Shepel, Natalia
Korovchuk, Olga
Kutsalo, Polina
Shegai, Petr
Kaprin, Andrei
author_facet Khvostunov, Igor K.
Nasonova, Elena
Krylov, Valeriy
Rodichev, Andrei
Kochetova, Tatiana
Shepel, Natalia
Korovchuk, Olga
Kutsalo, Polina
Shegai, Petr
Kaprin, Andrei
author_sort Khvostunov, Igor K.
collection PubMed
description The risk of toxicity attributable to radioiodine therapy (RIT) remains a subject of ongoing research, with a whole-body dose of 2 Gy proposed as a safe limit. This article evaluates the RIT-induced cytogenetic damage in two rare differentiated thyroid cancer (DTC) cases, including the first follow-up study of a pediatric DTC patient. Chromosome damage in the patient’s peripheral blood lymphocytes (PBL) was examined using conventional metaphase assay, painting of chromosomes 2, 4, and 12 (FISH), and multiplex fluorescence in situ hybridization (mFISH). Patient 1 (female, 1.6 y.o.) received four RIT courses over 1.1 years. Patient 2 (female, 49 y.o.) received 12 courses over 6.4 years, the last two of which were examined. Blood samples were collected before and 3–4 days after the treatment. Chromosome aberrations (CA) analyzed by conventional and FISH methods were converted to a whole-body dose accounting for the dose rate effect. The mFISH method showed an increase in total aberrant cell frequency following each RIT course, while cells carrying unstable aberrations predominated in the yield. The proportion of cells containing stable CA associated with long-term cytogenetic risk remained mostly unchanged during follow-up for both patients. A one-time administration of RIT was safe, as the threshold of 2 Gy for the whole-body dose was not exceeded. The risk of side effects projected from RIT-attributable cytogenetic damage was low, suggesting a good long-term prognosis. In rare cases, such as the ones reviewed in this study, individual planning based on cytogenetic biodosimetry is strongly recommended.
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spelling pubmed-100492722023-03-29 Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study Khvostunov, Igor K. Nasonova, Elena Krylov, Valeriy Rodichev, Andrei Kochetova, Tatiana Shepel, Natalia Korovchuk, Olga Kutsalo, Polina Shegai, Petr Kaprin, Andrei Int J Mol Sci Article The risk of toxicity attributable to radioiodine therapy (RIT) remains a subject of ongoing research, with a whole-body dose of 2 Gy proposed as a safe limit. This article evaluates the RIT-induced cytogenetic damage in two rare differentiated thyroid cancer (DTC) cases, including the first follow-up study of a pediatric DTC patient. Chromosome damage in the patient’s peripheral blood lymphocytes (PBL) was examined using conventional metaphase assay, painting of chromosomes 2, 4, and 12 (FISH), and multiplex fluorescence in situ hybridization (mFISH). Patient 1 (female, 1.6 y.o.) received four RIT courses over 1.1 years. Patient 2 (female, 49 y.o.) received 12 courses over 6.4 years, the last two of which were examined. Blood samples were collected before and 3–4 days after the treatment. Chromosome aberrations (CA) analyzed by conventional and FISH methods were converted to a whole-body dose accounting for the dose rate effect. The mFISH method showed an increase in total aberrant cell frequency following each RIT course, while cells carrying unstable aberrations predominated in the yield. The proportion of cells containing stable CA associated with long-term cytogenetic risk remained mostly unchanged during follow-up for both patients. A one-time administration of RIT was safe, as the threshold of 2 Gy for the whole-body dose was not exceeded. The risk of side effects projected from RIT-attributable cytogenetic damage was low, suggesting a good long-term prognosis. In rare cases, such as the ones reviewed in this study, individual planning based on cytogenetic biodosimetry is strongly recommended. MDPI 2023-03-07 /pmc/articles/PMC10049272/ /pubmed/36982202 http://dx.doi.org/10.3390/ijms24065128 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khvostunov, Igor K.
Nasonova, Elena
Krylov, Valeriy
Rodichev, Andrei
Kochetova, Tatiana
Shepel, Natalia
Korovchuk, Olga
Kutsalo, Polina
Shegai, Petr
Kaprin, Andrei
Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title_full Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title_fullStr Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title_full_unstemmed Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title_short Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study
title_sort cytogenetic damage induced by radioiodine therapy: a follow-up case study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049272/
https://www.ncbi.nlm.nih.gov/pubmed/36982202
http://dx.doi.org/10.3390/ijms24065128
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