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Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering

Developmental engineering (DE) aims to culture mammalian cells on corresponding modular scaffolds (scale: micron to millimeter), then assemble these into functional tissues imitating natural developmental biology processes. This research intended to investigate the influences of polymeric particles...

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Autores principales: Xiang, Yu, Yan, Jiongyi, Bao, Xujin, Gleadall, Andrew, Roach, Paul, Sun, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049291/
https://www.ncbi.nlm.nih.gov/pubmed/36982306
http://dx.doi.org/10.3390/ijms24065234
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author Xiang, Yu
Yan, Jiongyi
Bao, Xujin
Gleadall, Andrew
Roach, Paul
Sun, Tao
author_facet Xiang, Yu
Yan, Jiongyi
Bao, Xujin
Gleadall, Andrew
Roach, Paul
Sun, Tao
author_sort Xiang, Yu
collection PubMed
description Developmental engineering (DE) aims to culture mammalian cells on corresponding modular scaffolds (scale: micron to millimeter), then assemble these into functional tissues imitating natural developmental biology processes. This research intended to investigate the influences of polymeric particles on modular tissue cultures. When poly(methyl methacrylate) (PMMA), poly(lactic acid) (PLA) and polystyrene (PS) particles (diameter: 5–100 µm) were fabricated and submerged in culture medium in tissue culture plastics (TCPs) for modular tissue cultures, the majority of adjacent PMMA, some PLA but no PS particles aggregated. Human dermal fibroblasts (HDFs) could be directly seeded onto large (diameter: 30–100 µm) PMMA particles, but not small (diameter: 5–20 µm) PMMA, nor all the PLA and PS particles. During tissue cultures, HDFs migrated from the TCPs surfaces onto all the particles, while the clustered PMMA or PLA particles were colonized by HDFs into modular tissues with varying sizes. Further comparisons revealed that HDFs utilized the same cell bridging and stacking strategies to colonize single or clustered polymeric particles, and the finely controlled open pores, corners and gaps on 3D-printed PLA discs. These observed cell–scaffold interactions, which were then used to evaluate the adaptation of microcarrier-based cell expansion technologies for modular tissue manufacturing in DE.
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spelling pubmed-100492912023-03-29 Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering Xiang, Yu Yan, Jiongyi Bao, Xujin Gleadall, Andrew Roach, Paul Sun, Tao Int J Mol Sci Article Developmental engineering (DE) aims to culture mammalian cells on corresponding modular scaffolds (scale: micron to millimeter), then assemble these into functional tissues imitating natural developmental biology processes. This research intended to investigate the influences of polymeric particles on modular tissue cultures. When poly(methyl methacrylate) (PMMA), poly(lactic acid) (PLA) and polystyrene (PS) particles (diameter: 5–100 µm) were fabricated and submerged in culture medium in tissue culture plastics (TCPs) for modular tissue cultures, the majority of adjacent PMMA, some PLA but no PS particles aggregated. Human dermal fibroblasts (HDFs) could be directly seeded onto large (diameter: 30–100 µm) PMMA particles, but not small (diameter: 5–20 µm) PMMA, nor all the PLA and PS particles. During tissue cultures, HDFs migrated from the TCPs surfaces onto all the particles, while the clustered PMMA or PLA particles were colonized by HDFs into modular tissues with varying sizes. Further comparisons revealed that HDFs utilized the same cell bridging and stacking strategies to colonize single or clustered polymeric particles, and the finely controlled open pores, corners and gaps on 3D-printed PLA discs. These observed cell–scaffold interactions, which were then used to evaluate the adaptation of microcarrier-based cell expansion technologies for modular tissue manufacturing in DE. MDPI 2023-03-09 /pmc/articles/PMC10049291/ /pubmed/36982306 http://dx.doi.org/10.3390/ijms24065234 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiang, Yu
Yan, Jiongyi
Bao, Xujin
Gleadall, Andrew
Roach, Paul
Sun, Tao
Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title_full Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title_fullStr Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title_full_unstemmed Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title_short Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering
title_sort evaluation of polymeric particles for modular tissue cultures in developmental engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049291/
https://www.ncbi.nlm.nih.gov/pubmed/36982306
http://dx.doi.org/10.3390/ijms24065234
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