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Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae

Mitochondrial RTG (an acronym for ReTroGrade) signaling plays a cytoprotective role under various intracellular or environmental stresses. We have previously shown its contribution to osmoadaptation and capacity to sustain mitochondrial respiration in yeast. Here, we studied the interplay between RT...

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Autores principales: Di Noia, Maria Antonietta, Scarcia, Pasquale, Agrimi, Gennaro, Ocheja, Ohiemi Benjamin, Wahid, Ehtisham, Pisano, Isabella, Paradies, Eleonora, Palmieri, Luigi, Guaragnella, Cataldo, Guaragnella, Nicoletta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049445/
https://www.ncbi.nlm.nih.gov/pubmed/36982394
http://dx.doi.org/10.3390/ijms24065320
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author Di Noia, Maria Antonietta
Scarcia, Pasquale
Agrimi, Gennaro
Ocheja, Ohiemi Benjamin
Wahid, Ehtisham
Pisano, Isabella
Paradies, Eleonora
Palmieri, Luigi
Guaragnella, Cataldo
Guaragnella, Nicoletta
author_facet Di Noia, Maria Antonietta
Scarcia, Pasquale
Agrimi, Gennaro
Ocheja, Ohiemi Benjamin
Wahid, Ehtisham
Pisano, Isabella
Paradies, Eleonora
Palmieri, Luigi
Guaragnella, Cataldo
Guaragnella, Nicoletta
author_sort Di Noia, Maria Antonietta
collection PubMed
description Mitochondrial RTG (an acronym for ReTroGrade) signaling plays a cytoprotective role under various intracellular or environmental stresses. We have previously shown its contribution to osmoadaptation and capacity to sustain mitochondrial respiration in yeast. Here, we studied the interplay between RTG2, the main positive regulator of the RTG pathway, and HAP4, encoding the catalytic subunit of the Hap2-5 complex required for the expression of many mitochondrial proteins that function in the tricarboxylic acid (TCA) cycle and electron transport, upon osmotic stress. Cell growth features, mitochondrial respiratory competence, retrograde signaling activation, and TCA cycle gene expression were comparatively evaluated in wild type and mutant cells in the presence and in the absence of salt stress. We showed that the inactivation of HAP4 improved the kinetics of osmoadaptation by eliciting both the activation of retrograde signaling and the upregulation of three TCA cycle genes: citrate synthase 1 (CIT1), aconitase 1 (ACO1), and isocitrate dehydrogenase 1 (IDH1). Interestingly, their increased expression was mostly dependent on RTG2. Impaired respiratory competence in the HAP4 mutant does not affect its faster adaptive response to stress. These findings indicate that the involvement of the RTG pathway in osmostress is fostered in a cellular context of constitutively reduced respiratory capacity. Moreover, it is evident that the RTG pathway mediates peroxisomes–mitochondria communication by modulating the metabolic function of mitochondria in osmoadaptation.
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spelling pubmed-100494452023-03-29 Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae Di Noia, Maria Antonietta Scarcia, Pasquale Agrimi, Gennaro Ocheja, Ohiemi Benjamin Wahid, Ehtisham Pisano, Isabella Paradies, Eleonora Palmieri, Luigi Guaragnella, Cataldo Guaragnella, Nicoletta Int J Mol Sci Article Mitochondrial RTG (an acronym for ReTroGrade) signaling plays a cytoprotective role under various intracellular or environmental stresses. We have previously shown its contribution to osmoadaptation and capacity to sustain mitochondrial respiration in yeast. Here, we studied the interplay between RTG2, the main positive regulator of the RTG pathway, and HAP4, encoding the catalytic subunit of the Hap2-5 complex required for the expression of many mitochondrial proteins that function in the tricarboxylic acid (TCA) cycle and electron transport, upon osmotic stress. Cell growth features, mitochondrial respiratory competence, retrograde signaling activation, and TCA cycle gene expression were comparatively evaluated in wild type and mutant cells in the presence and in the absence of salt stress. We showed that the inactivation of HAP4 improved the kinetics of osmoadaptation by eliciting both the activation of retrograde signaling and the upregulation of three TCA cycle genes: citrate synthase 1 (CIT1), aconitase 1 (ACO1), and isocitrate dehydrogenase 1 (IDH1). Interestingly, their increased expression was mostly dependent on RTG2. Impaired respiratory competence in the HAP4 mutant does not affect its faster adaptive response to stress. These findings indicate that the involvement of the RTG pathway in osmostress is fostered in a cellular context of constitutively reduced respiratory capacity. Moreover, it is evident that the RTG pathway mediates peroxisomes–mitochondria communication by modulating the metabolic function of mitochondria in osmoadaptation. MDPI 2023-03-10 /pmc/articles/PMC10049445/ /pubmed/36982394 http://dx.doi.org/10.3390/ijms24065320 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Noia, Maria Antonietta
Scarcia, Pasquale
Agrimi, Gennaro
Ocheja, Ohiemi Benjamin
Wahid, Ehtisham
Pisano, Isabella
Paradies, Eleonora
Palmieri, Luigi
Guaragnella, Cataldo
Guaragnella, Nicoletta
Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title_full Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title_fullStr Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title_full_unstemmed Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title_short Inactivation of HAP4 Accelerates RTG-Dependent Osmoadaptation in Saccharomyces cerevisiae
title_sort inactivation of hap4 accelerates rtg-dependent osmoadaptation in saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049445/
https://www.ncbi.nlm.nih.gov/pubmed/36982394
http://dx.doi.org/10.3390/ijms24065320
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