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Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study

Vitamin D plays an important role in numerous cellular functions due to the ability to bind the Vitamin D receptor (VDR), which is present in different tissues. Several human diseases depend on low vitamin D3 (human isoform) serum level, and supplementation is necessary. However, vitamin D3 has poor...

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Autores principales: Uberti, Francesca, Trotta, Francesco, Pagliaro, Pasquale, Bisericaru, Daniel Mihai, Cavalli, Roberta, Ferrari, Sara, Penna, Claudia, Matencio, Adrián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049479/
https://www.ncbi.nlm.nih.gov/pubmed/36982396
http://dx.doi.org/10.3390/ijms24065322
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author Uberti, Francesca
Trotta, Francesco
Pagliaro, Pasquale
Bisericaru, Daniel Mihai
Cavalli, Roberta
Ferrari, Sara
Penna, Claudia
Matencio, Adrián
author_facet Uberti, Francesca
Trotta, Francesco
Pagliaro, Pasquale
Bisericaru, Daniel Mihai
Cavalli, Roberta
Ferrari, Sara
Penna, Claudia
Matencio, Adrián
author_sort Uberti, Francesca
collection PubMed
description Vitamin D plays an important role in numerous cellular functions due to the ability to bind the Vitamin D receptor (VDR), which is present in different tissues. Several human diseases depend on low vitamin D3 (human isoform) serum level, and supplementation is necessary. However, vitamin D3 has poor bioavailability, and several strategies are tested to increase its absorption. In this work, the complexation of vitamin D3 in Cyclodextrin-based nanosponge (CD-NS, in particular, βNS-CDI 1:4) was carried out to study the possible enhancement of bioactivity. The βNS-CDI 1:4 was synthesized by mechanochemistry, and the complex was confirmed using FTIR-ATR and TGA. TGA demonstrated higher thermostability of the complexed form. Subsequently, in vitro experiments were performed to evaluate the biological activity of Vitamin D3 complexed in the nanosponges on intestinal cells and assess its bioavailability without cytotoxic effect. The Vitamin D3 complexes enhance cellular activity at the intestinal level and improve its bioavailability. In conclusion, this study demonstrates for the first time the ability of CD-NS complexes to improve the chemical and biological function of Vitamin D3.
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spelling pubmed-100494792023-03-29 Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study Uberti, Francesca Trotta, Francesco Pagliaro, Pasquale Bisericaru, Daniel Mihai Cavalli, Roberta Ferrari, Sara Penna, Claudia Matencio, Adrián Int J Mol Sci Article Vitamin D plays an important role in numerous cellular functions due to the ability to bind the Vitamin D receptor (VDR), which is present in different tissues. Several human diseases depend on low vitamin D3 (human isoform) serum level, and supplementation is necessary. However, vitamin D3 has poor bioavailability, and several strategies are tested to increase its absorption. In this work, the complexation of vitamin D3 in Cyclodextrin-based nanosponge (CD-NS, in particular, βNS-CDI 1:4) was carried out to study the possible enhancement of bioactivity. The βNS-CDI 1:4 was synthesized by mechanochemistry, and the complex was confirmed using FTIR-ATR and TGA. TGA demonstrated higher thermostability of the complexed form. Subsequently, in vitro experiments were performed to evaluate the biological activity of Vitamin D3 complexed in the nanosponges on intestinal cells and assess its bioavailability without cytotoxic effect. The Vitamin D3 complexes enhance cellular activity at the intestinal level and improve its bioavailability. In conclusion, this study demonstrates for the first time the ability of CD-NS complexes to improve the chemical and biological function of Vitamin D3. MDPI 2023-03-10 /pmc/articles/PMC10049479/ /pubmed/36982396 http://dx.doi.org/10.3390/ijms24065322 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Uberti, Francesca
Trotta, Francesco
Pagliaro, Pasquale
Bisericaru, Daniel Mihai
Cavalli, Roberta
Ferrari, Sara
Penna, Claudia
Matencio, Adrián
Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title_full Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title_fullStr Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title_full_unstemmed Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title_short Developing New Cyclodextrin-Based Nanosponges Complexes to Improve Vitamin D Absorption in an In Vitro Study
title_sort developing new cyclodextrin-based nanosponges complexes to improve vitamin d absorption in an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049479/
https://www.ncbi.nlm.nih.gov/pubmed/36982396
http://dx.doi.org/10.3390/ijms24065322
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