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Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes

Early pregnancy modulates the maternal immune system, including the spleen and lymph nodes, which participate in maternal innate and adaptive immune responses. Methods: Ovine spleens and lymph nodes were sampled at day 16 of the estrous cycle, and at days 13, 16 and 25 of gestation, and qRT-PCR, Wes...

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Autores principales: Fang, Shengya, Cai, Chunjiang, Bai, Ying, Zhang, Leying, Yang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049502/
https://www.ncbi.nlm.nih.gov/pubmed/36982231
http://dx.doi.org/10.3390/ijms24065156
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author Fang, Shengya
Cai, Chunjiang
Bai, Ying
Zhang, Leying
Yang, Ling
author_facet Fang, Shengya
Cai, Chunjiang
Bai, Ying
Zhang, Leying
Yang, Ling
author_sort Fang, Shengya
collection PubMed
description Early pregnancy modulates the maternal immune system, including the spleen and lymph nodes, which participate in maternal innate and adaptive immune responses. Methods: Ovine spleens and lymph nodes were sampled at day 16 of the estrous cycle, and at days 13, 16 and 25 of gestation, and qRT-PCR, Western blot and immunohistochemistry analysis were used to analyze the expression of the IκB family, including BCL-3, IκBα, IκBβ, IκBε, IKKγ, IκBNS and IκBζ. Early pregnancy induced expression of BCL-3, IκBα, IκBε, IKKγ and IκBζ, and expression of BCL-3, IκBβ and IκBNS peaked at day 16 of pregnancy in the spleen. However, early pregnancy suppressed the expression of BCL-3 and IκBNS, but stimulated the expression of IκBβ and IκBζ, and expression levels of IκBα, IκBβ, IκBε and IKKγ peaked in lymph nodes at days 13 and/or 16 of pregnancy. Early pregnancy changed the expression of the IκB family in the maternal spleen and lymph node in a tissue-specific manner, suggesting that the modulation of the IκB family may be involved in regulation of maternal functions of the spleen and lymph nodes, which are necessary for the establishment of maternal immune tolerance during early pregnancy in sheep.
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spelling pubmed-100495022023-03-29 Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes Fang, Shengya Cai, Chunjiang Bai, Ying Zhang, Leying Yang, Ling Int J Mol Sci Article Early pregnancy modulates the maternal immune system, including the spleen and lymph nodes, which participate in maternal innate and adaptive immune responses. Methods: Ovine spleens and lymph nodes were sampled at day 16 of the estrous cycle, and at days 13, 16 and 25 of gestation, and qRT-PCR, Western blot and immunohistochemistry analysis were used to analyze the expression of the IκB family, including BCL-3, IκBα, IκBβ, IκBε, IKKγ, IκBNS and IκBζ. Early pregnancy induced expression of BCL-3, IκBα, IκBε, IKKγ and IκBζ, and expression of BCL-3, IκBβ and IκBNS peaked at day 16 of pregnancy in the spleen. However, early pregnancy suppressed the expression of BCL-3 and IκBNS, but stimulated the expression of IκBβ and IκBζ, and expression levels of IκBα, IκBβ, IκBε and IKKγ peaked in lymph nodes at days 13 and/or 16 of pregnancy. Early pregnancy changed the expression of the IκB family in the maternal spleen and lymph node in a tissue-specific manner, suggesting that the modulation of the IκB family may be involved in regulation of maternal functions of the spleen and lymph nodes, which are necessary for the establishment of maternal immune tolerance during early pregnancy in sheep. MDPI 2023-03-08 /pmc/articles/PMC10049502/ /pubmed/36982231 http://dx.doi.org/10.3390/ijms24065156 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fang, Shengya
Cai, Chunjiang
Bai, Ying
Zhang, Leying
Yang, Ling
Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title_full Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title_fullStr Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title_full_unstemmed Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title_short Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes
title_sort early pregnancy regulates expression of ikappab family in ovine spleen and lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049502/
https://www.ncbi.nlm.nih.gov/pubmed/36982231
http://dx.doi.org/10.3390/ijms24065156
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