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The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice

The cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many restorer-of-fertility (Rf) genes have been characterized in various species over the decades, but more detailed work is needed...

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Autores principales: Zhao, Weibo, Geng, Han, Dan, Zhiwu, Zeng, Yafei, Wang, Mingyue, Xu, Wuwu, Hu, Zhongli, Huang, Wenchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049570/
https://www.ncbi.nlm.nih.gov/pubmed/36982518
http://dx.doi.org/10.3390/ijms24065442
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author Zhao, Weibo
Geng, Han
Dan, Zhiwu
Zeng, Yafei
Wang, Mingyue
Xu, Wuwu
Hu, Zhongli
Huang, Wenchao
author_facet Zhao, Weibo
Geng, Han
Dan, Zhiwu
Zeng, Yafei
Wang, Mingyue
Xu, Wuwu
Hu, Zhongli
Huang, Wenchao
author_sort Zhao, Weibo
collection PubMed
description The cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many restorer-of-fertility (Rf) genes have been characterized in various species over the decades, but more detailed work is needed to investigate the fertility restoration mechanism. Here, we identified an alpha subunit of mitochondrial processing peptidase (MPPA) that is involved in the fertility restoration process in Honglian-CMS rice. MPPA is a mitochondrial localized protein and interacted with the RF6 protein encoded by the Rf6. MPPA indirectly interacted with hexokinase 6, namely another partner of RF6, to form a protein complex with the same molecular weight as the mitochondrial F(1)F(0)-ATP synthase in processing the CMS transcript. Loss-of-function of MPPA resulted in a defect in pollen fertility, the mppa(+/−) heterozygotes showed semi-sterility phenotype and the accumulation of CMS-associated protein ORFH79, showing restrained processing of the CMS-associated atp6-OrfH79 in the mutant plant. Taken together, these results threw new light on the process of fertility restoration by investigating the RF6 fertility restoration complex. They also reveal the connections between signal peptide cleavage and the fertility restoration process in Honglian-CMS rice.
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spelling pubmed-100495702023-03-29 The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice Zhao, Weibo Geng, Han Dan, Zhiwu Zeng, Yafei Wang, Mingyue Xu, Wuwu Hu, Zhongli Huang, Wenchao Int J Mol Sci Article The cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration system is a favorable tool for the utilization of heterosis in plant hybrid breeding. Many restorer-of-fertility (Rf) genes have been characterized in various species over the decades, but more detailed work is needed to investigate the fertility restoration mechanism. Here, we identified an alpha subunit of mitochondrial processing peptidase (MPPA) that is involved in the fertility restoration process in Honglian-CMS rice. MPPA is a mitochondrial localized protein and interacted with the RF6 protein encoded by the Rf6. MPPA indirectly interacted with hexokinase 6, namely another partner of RF6, to form a protein complex with the same molecular weight as the mitochondrial F(1)F(0)-ATP synthase in processing the CMS transcript. Loss-of-function of MPPA resulted in a defect in pollen fertility, the mppa(+/−) heterozygotes showed semi-sterility phenotype and the accumulation of CMS-associated protein ORFH79, showing restrained processing of the CMS-associated atp6-OrfH79 in the mutant plant. Taken together, these results threw new light on the process of fertility restoration by investigating the RF6 fertility restoration complex. They also reveal the connections between signal peptide cleavage and the fertility restoration process in Honglian-CMS rice. MDPI 2023-03-13 /pmc/articles/PMC10049570/ /pubmed/36982518 http://dx.doi.org/10.3390/ijms24065442 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Weibo
Geng, Han
Dan, Zhiwu
Zeng, Yafei
Wang, Mingyue
Xu, Wuwu
Hu, Zhongli
Huang, Wenchao
The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title_full The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title_fullStr The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title_full_unstemmed The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title_short The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice
title_sort alpha subunit of mitochondrial processing peptidase participated in fertility restoration in honglian-cms rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049570/
https://www.ncbi.nlm.nih.gov/pubmed/36982518
http://dx.doi.org/10.3390/ijms24065442
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