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Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research

Immortalized cell lines are widely used in vitro tools in oncology and hematology research. While these cell lines represent artificial systems and may accumulate genetic aberrations with each passage, they are still considered valuable models for pilot, preliminary, and screening studies. Despite t...

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Autores principales: Skopek, Rafał, Palusińska, Małgorzata, Kaczor-Keller, Katarzyna, Pingwara, Rafał, Papierniak-Wyglądała, Anna, Schenk, Tino, Lewicki, Sławomir, Zelent, Artur, Szymański, Łukasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049680/
https://www.ncbi.nlm.nih.gov/pubmed/36982453
http://dx.doi.org/10.3390/ijms24065377
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author Skopek, Rafał
Palusińska, Małgorzata
Kaczor-Keller, Katarzyna
Pingwara, Rafał
Papierniak-Wyglądała, Anna
Schenk, Tino
Lewicki, Sławomir
Zelent, Artur
Szymański, Łukasz
author_facet Skopek, Rafał
Palusińska, Małgorzata
Kaczor-Keller, Katarzyna
Pingwara, Rafał
Papierniak-Wyglądała, Anna
Schenk, Tino
Lewicki, Sławomir
Zelent, Artur
Szymański, Łukasz
author_sort Skopek, Rafał
collection PubMed
description Immortalized cell lines are widely used in vitro tools in oncology and hematology research. While these cell lines represent artificial systems and may accumulate genetic aberrations with each passage, they are still considered valuable models for pilot, preliminary, and screening studies. Despite their limitations, cell lines are cost-effective and provide repeatable and comparable results. Choosing the appropriate cell line for acute myeloid leukemia (AML) research is crucial for obtaining reliable and relevant results. Several factors should be considered when selecting a cell line for AML research, such as specific markers and genetic abnormalities associated with different subtypes of AML. It is also essential to evaluate the karyotype and mutational profile of the cell line, as these can influence the behavior and response to the treatment of the cells. In this review, we evaluate immortalized AML cell lines and discuss the issues surrounding them concerning the revised World Health Organization and the French–American–British classifications.
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spelling pubmed-100496802023-03-29 Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research Skopek, Rafał Palusińska, Małgorzata Kaczor-Keller, Katarzyna Pingwara, Rafał Papierniak-Wyglądała, Anna Schenk, Tino Lewicki, Sławomir Zelent, Artur Szymański, Łukasz Int J Mol Sci Review Immortalized cell lines are widely used in vitro tools in oncology and hematology research. While these cell lines represent artificial systems and may accumulate genetic aberrations with each passage, they are still considered valuable models for pilot, preliminary, and screening studies. Despite their limitations, cell lines are cost-effective and provide repeatable and comparable results. Choosing the appropriate cell line for acute myeloid leukemia (AML) research is crucial for obtaining reliable and relevant results. Several factors should be considered when selecting a cell line for AML research, such as specific markers and genetic abnormalities associated with different subtypes of AML. It is also essential to evaluate the karyotype and mutational profile of the cell line, as these can influence the behavior and response to the treatment of the cells. In this review, we evaluate immortalized AML cell lines and discuss the issues surrounding them concerning the revised World Health Organization and the French–American–British classifications. MDPI 2023-03-11 /pmc/articles/PMC10049680/ /pubmed/36982453 http://dx.doi.org/10.3390/ijms24065377 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Skopek, Rafał
Palusińska, Małgorzata
Kaczor-Keller, Katarzyna
Pingwara, Rafał
Papierniak-Wyglądała, Anna
Schenk, Tino
Lewicki, Sławomir
Zelent, Artur
Szymański, Łukasz
Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title_full Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title_fullStr Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title_full_unstemmed Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title_short Choosing the Right Cell Line for Acute Myeloid Leukemia (AML) Research
title_sort choosing the right cell line for acute myeloid leukemia (aml) research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049680/
https://www.ncbi.nlm.nih.gov/pubmed/36982453
http://dx.doi.org/10.3390/ijms24065377
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