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The Effect of Centrifuge Duration on Fat Graft Survival
Background Although fat grafts are widely used for reconstruction and aesthetic purposes, their survival rates differ significantly. Centrifugation is one of the methods used to increase the survival of fat grafts. However, experimental studies examining the long-term outcomes of centrifugation dur...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049827/ https://www.ncbi.nlm.nih.gov/pubmed/36998923 http://dx.doi.org/10.1055/s-0042-1759495 |
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author | Demirel, Oguzhan Karakol, Percin Bozkurt, Mehmet Tuglu, Mehmet Ibrahim |
author_facet | Demirel, Oguzhan Karakol, Percin Bozkurt, Mehmet Tuglu, Mehmet Ibrahim |
author_sort | Demirel, Oguzhan |
collection | PubMed |
description | Background Although fat grafts are widely used for reconstruction and aesthetic purposes, their survival rates differ significantly. Centrifugation is one of the methods used to increase the survival of fat grafts. However, experimental studies examining the long-term outcomes of centrifugation duration are currently limited. Thus, in the present study, the effects of centrifugation duration on the survival of fat grafts were assessed using an animal model. Methods Thirty Sprague Dawley rats were included in the study and fat grafts were obtained from each specimen by excision from inguinal fat pads. Preparation protocols were administered as an en-bloc fat graft in Group 1, minced fat graft in Group 2, and fat graft centrifuged at 1,054 ×g for 2 minutes, 3 minutes, and 4 minutes in Group 3, 4, and 5, respectively. After 12 weeks of follow-up, grafts were harvested and were subjected to histopathological evaluation based on an established scoring system. Results En-block fat grafts were associated with necrosis, fibrosis, inflammation, vacuole formation, and alterations in adipocyte morphology. Among the three centrifugation groups, Group 3 demonstrated the best adipocyte viability and vascularity. However, graft weights decreased in all experimental groups. Conclusion The centrifugation process may have positive effects on adipocyte survival by means of purifying the fat graft and increasing adipocyte concentration. When the centrifugal durations were compared, 3-minute centrifuge yielded the most favorable results. |
format | Online Article Text |
id | pubmed-10049827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100498272023-03-29 The Effect of Centrifuge Duration on Fat Graft Survival Demirel, Oguzhan Karakol, Percin Bozkurt, Mehmet Tuglu, Mehmet Ibrahim Indian J Plast Surg Background Although fat grafts are widely used for reconstruction and aesthetic purposes, their survival rates differ significantly. Centrifugation is one of the methods used to increase the survival of fat grafts. However, experimental studies examining the long-term outcomes of centrifugation duration are currently limited. Thus, in the present study, the effects of centrifugation duration on the survival of fat grafts were assessed using an animal model. Methods Thirty Sprague Dawley rats were included in the study and fat grafts were obtained from each specimen by excision from inguinal fat pads. Preparation protocols were administered as an en-bloc fat graft in Group 1, minced fat graft in Group 2, and fat graft centrifuged at 1,054 ×g for 2 minutes, 3 minutes, and 4 minutes in Group 3, 4, and 5, respectively. After 12 weeks of follow-up, grafts were harvested and were subjected to histopathological evaluation based on an established scoring system. Results En-block fat grafts were associated with necrosis, fibrosis, inflammation, vacuole formation, and alterations in adipocyte morphology. Among the three centrifugation groups, Group 3 demonstrated the best adipocyte viability and vascularity. However, graft weights decreased in all experimental groups. Conclusion The centrifugation process may have positive effects on adipocyte survival by means of purifying the fat graft and increasing adipocyte concentration. When the centrifugal durations were compared, 3-minute centrifuge yielded the most favorable results. Thieme Medical and Scientific Publishers Pvt. Ltd. 2022-12-22 /pmc/articles/PMC10049827/ /pubmed/36998923 http://dx.doi.org/10.1055/s-0042-1759495 Text en Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Demirel, Oguzhan Karakol, Percin Bozkurt, Mehmet Tuglu, Mehmet Ibrahim The Effect of Centrifuge Duration on Fat Graft Survival |
title | The Effect of Centrifuge Duration on Fat Graft Survival |
title_full | The Effect of Centrifuge Duration on Fat Graft Survival |
title_fullStr | The Effect of Centrifuge Duration on Fat Graft Survival |
title_full_unstemmed | The Effect of Centrifuge Duration on Fat Graft Survival |
title_short | The Effect of Centrifuge Duration on Fat Graft Survival |
title_sort | effect of centrifuge duration on fat graft survival |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049827/ https://www.ncbi.nlm.nih.gov/pubmed/36998923 http://dx.doi.org/10.1055/s-0042-1759495 |
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