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Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development

The Gly-Asp-Ser-Leu (GDSL)-type esterase/lipase proteins (GELP) are one of the most important families of lipolytic enzymes and play prominent roles in seed germination and early seedling establishment through mobilizing the lipids stored in seeds. However, there are no comprehensive studies systema...

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Autores principales: Ding, Yahui, Xing, Liwen, Xu, Jiamin, Jiang, Teng, Tang, Xiuhua, Wang, Yaxuan, Huang, Shuhua, Hao, Wenfang, Zhou, Xiaona, Zhang, Yanfeng, Xie, Chang Gen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050346/
https://www.ncbi.nlm.nih.gov/pubmed/37008509
http://dx.doi.org/10.3389/fpls.2023.1139972
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author Ding, Yahui
Xing, Liwen
Xu, Jiamin
Jiang, Teng
Tang, Xiuhua
Wang, Yaxuan
Huang, Shuhua
Hao, Wenfang
Zhou, Xiaona
Zhang, Yanfeng
Xie, Chang Gen
author_facet Ding, Yahui
Xing, Liwen
Xu, Jiamin
Jiang, Teng
Tang, Xiuhua
Wang, Yaxuan
Huang, Shuhua
Hao, Wenfang
Zhou, Xiaona
Zhang, Yanfeng
Xie, Chang Gen
author_sort Ding, Yahui
collection PubMed
description The Gly-Asp-Ser-Leu (GDSL)-type esterase/lipase proteins (GELP) are one of the most important families of lipolytic enzymes and play prominent roles in seed germination and early seedling establishment through mobilizing the lipids stored in seeds. However, there are no comprehensive studies systematically investigating the GELP gene family in Brassica napus (BnGELP), and their biological significance to these physiological processes are far from understood. In the present study, a total of 240 BnGELP genes were identified in B. napus cultivar “Zhongshuang 11” (ZS11), which is nearly 2.3-fold more GELP genes than in Arabidopsis thaliana. The BnGELP genes clustered into 5 clades based on phylogenetic analysis. Ten BnGELPs were identified through zymogram analysis of esterase activity followed by mass spectrometry, among which five clustered into the clade 5. Gene and protein architecture, gene expression, and cis-element analyses of BnGELP genes in clade 5 suggested that they may play different roles in different tissues and in response to different abiotic stresses. BnGELP99 and BnGELP159 were slightly induced by cold, which may be attributed to two low-temperature responsive cis-acting regulatory elements present in their promoters. An increased activity of esterase isozymes by cold was also observed, which may reflect other cold inducible esterases/lipases in addition to the ten identified BnGELPs. This study provides a systemic view of the BnGELP gene family and offers a strategy for researchers to identify candidate esterase/lipase genes responsible for lipid mobilization during seed germination and early seedling establishment.
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spelling pubmed-100503462023-03-30 Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development Ding, Yahui Xing, Liwen Xu, Jiamin Jiang, Teng Tang, Xiuhua Wang, Yaxuan Huang, Shuhua Hao, Wenfang Zhou, Xiaona Zhang, Yanfeng Xie, Chang Gen Front Plant Sci Plant Science The Gly-Asp-Ser-Leu (GDSL)-type esterase/lipase proteins (GELP) are one of the most important families of lipolytic enzymes and play prominent roles in seed germination and early seedling establishment through mobilizing the lipids stored in seeds. However, there are no comprehensive studies systematically investigating the GELP gene family in Brassica napus (BnGELP), and their biological significance to these physiological processes are far from understood. In the present study, a total of 240 BnGELP genes were identified in B. napus cultivar “Zhongshuang 11” (ZS11), which is nearly 2.3-fold more GELP genes than in Arabidopsis thaliana. The BnGELP genes clustered into 5 clades based on phylogenetic analysis. Ten BnGELPs were identified through zymogram analysis of esterase activity followed by mass spectrometry, among which five clustered into the clade 5. Gene and protein architecture, gene expression, and cis-element analyses of BnGELP genes in clade 5 suggested that they may play different roles in different tissues and in response to different abiotic stresses. BnGELP99 and BnGELP159 were slightly induced by cold, which may be attributed to two low-temperature responsive cis-acting regulatory elements present in their promoters. An increased activity of esterase isozymes by cold was also observed, which may reflect other cold inducible esterases/lipases in addition to the ten identified BnGELPs. This study provides a systemic view of the BnGELP gene family and offers a strategy for researchers to identify candidate esterase/lipase genes responsible for lipid mobilization during seed germination and early seedling establishment. Frontiers Media S.A. 2023-03-15 /pmc/articles/PMC10050346/ /pubmed/37008509 http://dx.doi.org/10.3389/fpls.2023.1139972 Text en Copyright © 2023 Ding, Xing, Xu, Jiang, Tang, Wang, Huang, Hao, Zhou, Zhang and Xie https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ding, Yahui
Xing, Liwen
Xu, Jiamin
Jiang, Teng
Tang, Xiuhua
Wang, Yaxuan
Huang, Shuhua
Hao, Wenfang
Zhou, Xiaona
Zhang, Yanfeng
Xie, Chang Gen
Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title_full Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title_fullStr Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title_full_unstemmed Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title_short Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development
title_sort genome-wide exploration of the gdsl-type esterase/lipase gene family in rapeseed reveals several bngelp proteins active during early seedling development
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050346/
https://www.ncbi.nlm.nih.gov/pubmed/37008509
http://dx.doi.org/10.3389/fpls.2023.1139972
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