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Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050704/ https://www.ncbi.nlm.nih.gov/pubmed/37008006 http://dx.doi.org/10.3389/fphys.2023.1123583 |
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author | Li, Kailong Liu, Kanghong Wang, Xing Ma, Mingyong Luo, Xiangwen Chen, Wuying Chen, Ang Peng, Zhaopu Zhang, Deyong |
author_facet | Li, Kailong Liu, Kanghong Wang, Xing Ma, Mingyong Luo, Xiangwen Chen, Wuying Chen, Ang Peng, Zhaopu Zhang, Deyong |
author_sort | Li, Kailong |
collection | PubMed |
description | The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in the nymph stages by ecdysone signaling. Transcription disruption of NlHR3 and NlFTZ-F1 expression prevents nymph ecdysis and metamorphosis, which leads to abnormal appearance, malformed ovaries, and lethal phenotypes. In addition, we demonstrate that NlHR3 and NlFTZ-F1 regulate molting and reproduction by interacting with the intrinsic 20E and JH signaling pathways. Our work offers a deep insight into the action mechanisms of HR3 and FTZ-F1 in insects. Moreover, NlHR3 and NlFTZ-F1 could properly be exploited as potential target genes for developing RNAi-based pesticides to control N. lugens. |
format | Online Article Text |
id | pubmed-10050704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100507042023-03-30 Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) Li, Kailong Liu, Kanghong Wang, Xing Ma, Mingyong Luo, Xiangwen Chen, Wuying Chen, Ang Peng, Zhaopu Zhang, Deyong Front Physiol Physiology The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in the nymph stages by ecdysone signaling. Transcription disruption of NlHR3 and NlFTZ-F1 expression prevents nymph ecdysis and metamorphosis, which leads to abnormal appearance, malformed ovaries, and lethal phenotypes. In addition, we demonstrate that NlHR3 and NlFTZ-F1 regulate molting and reproduction by interacting with the intrinsic 20E and JH signaling pathways. Our work offers a deep insight into the action mechanisms of HR3 and FTZ-F1 in insects. Moreover, NlHR3 and NlFTZ-F1 could properly be exploited as potential target genes for developing RNAi-based pesticides to control N. lugens. Frontiers Media S.A. 2023-03-15 /pmc/articles/PMC10050704/ /pubmed/37008006 http://dx.doi.org/10.3389/fphys.2023.1123583 Text en Copyright © 2023 Li, Liu, Wang, Ma, Luo, Chen, Chen, Peng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Li, Kailong Liu, Kanghong Wang, Xing Ma, Mingyong Luo, Xiangwen Chen, Wuying Chen, Ang Peng, Zhaopu Zhang, Deyong Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title | Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title_full | Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title_fullStr | Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title_full_unstemmed | Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title_short | Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) |
title_sort | role of nuclear receptors nlhr3 and nlftz-f1 in regulating molting and reproduction in nilaparvata lugens (stål) |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050704/ https://www.ncbi.nlm.nih.gov/pubmed/37008006 http://dx.doi.org/10.3389/fphys.2023.1123583 |
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