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Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)

The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in th...

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Autores principales: Li, Kailong, Liu, Kanghong, Wang, Xing, Ma, Mingyong, Luo, Xiangwen, Chen, Wuying, Chen, Ang, Peng, Zhaopu, Zhang, Deyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050704/
https://www.ncbi.nlm.nih.gov/pubmed/37008006
http://dx.doi.org/10.3389/fphys.2023.1123583
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author Li, Kailong
Liu, Kanghong
Wang, Xing
Ma, Mingyong
Luo, Xiangwen
Chen, Wuying
Chen, Ang
Peng, Zhaopu
Zhang, Deyong
author_facet Li, Kailong
Liu, Kanghong
Wang, Xing
Ma, Mingyong
Luo, Xiangwen
Chen, Wuying
Chen, Ang
Peng, Zhaopu
Zhang, Deyong
author_sort Li, Kailong
collection PubMed
description The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in the nymph stages by ecdysone signaling. Transcription disruption of NlHR3 and NlFTZ-F1 expression prevents nymph ecdysis and metamorphosis, which leads to abnormal appearance, malformed ovaries, and lethal phenotypes. In addition, we demonstrate that NlHR3 and NlFTZ-F1 regulate molting and reproduction by interacting with the intrinsic 20E and JH signaling pathways. Our work offers a deep insight into the action mechanisms of HR3 and FTZ-F1 in insects. Moreover, NlHR3 and NlFTZ-F1 could properly be exploited as potential target genes for developing RNAi-based pesticides to control N. lugens.
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spelling pubmed-100507042023-03-30 Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål) Li, Kailong Liu, Kanghong Wang, Xing Ma, Mingyong Luo, Xiangwen Chen, Wuying Chen, Ang Peng, Zhaopu Zhang, Deyong Front Physiol Physiology The nuclear receptors HR3 and FTZ-F1 are highly conserved and function to regulate molting and reproduction in both hemimetabolous and holometabolous insects. However, their roles in Nilaparvata lugens are largely unknown. In the present study, we discover that NlHR3 and NlFTZ-F1 are activated in the nymph stages by ecdysone signaling. Transcription disruption of NlHR3 and NlFTZ-F1 expression prevents nymph ecdysis and metamorphosis, which leads to abnormal appearance, malformed ovaries, and lethal phenotypes. In addition, we demonstrate that NlHR3 and NlFTZ-F1 regulate molting and reproduction by interacting with the intrinsic 20E and JH signaling pathways. Our work offers a deep insight into the action mechanisms of HR3 and FTZ-F1 in insects. Moreover, NlHR3 and NlFTZ-F1 could properly be exploited as potential target genes for developing RNAi-based pesticides to control N. lugens. Frontiers Media S.A. 2023-03-15 /pmc/articles/PMC10050704/ /pubmed/37008006 http://dx.doi.org/10.3389/fphys.2023.1123583 Text en Copyright © 2023 Li, Liu, Wang, Ma, Luo, Chen, Chen, Peng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Li, Kailong
Liu, Kanghong
Wang, Xing
Ma, Mingyong
Luo, Xiangwen
Chen, Wuying
Chen, Ang
Peng, Zhaopu
Zhang, Deyong
Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title_full Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title_fullStr Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title_full_unstemmed Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title_short Role of nuclear receptors NlHR3 and NlFTZ-F1 in regulating molting and reproduction in Nilaparvata lugens (stål)
title_sort role of nuclear receptors nlhr3 and nlftz-f1 in regulating molting and reproduction in nilaparvata lugens (stål)
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050704/
https://www.ncbi.nlm.nih.gov/pubmed/37008006
http://dx.doi.org/10.3389/fphys.2023.1123583
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