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Large animal models for cardiac remuscularization studies: A methodological review

Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have...

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Autores principales: Yu, Yuexin, Tham, Seng Kong, Roslan, Fatin Fazrina, Shaharuddin, Bakiah, Yong, Yoke Keong, Guo, Zhikun, Tan, Jun Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050756/
https://www.ncbi.nlm.nih.gov/pubmed/37008331
http://dx.doi.org/10.3389/fcvm.2023.1011880
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author Yu, Yuexin
Tham, Seng Kong
Roslan, Fatin Fazrina
Shaharuddin, Bakiah
Yong, Yoke Keong
Guo, Zhikun
Tan, Jun Jie
author_facet Yu, Yuexin
Tham, Seng Kong
Roslan, Fatin Fazrina
Shaharuddin, Bakiah
Yong, Yoke Keong
Guo, Zhikun
Tan, Jun Jie
author_sort Yu, Yuexin
collection PubMed
description Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have proven the ability to derive specific and functional cardiomyocytes in large quantities for therapy. To test the remuscularization hypothesis, the strategy to model the disease in animals must resemble the pathophysiological conditions of myocardial infarction as in humans, to enable thorough testing of the safety and efficacy of the cardiomyocyte therapy before embarking on human trials. Rigorous experiments and in vivo findings using large mammals are increasingly important to simulate clinical reality and increase translatability into clinical practice. Hence, this review focus on large animal models which have been used in cardiac remuscularization studies using cardiomyocytes derived from human pluripotent stem cells. The commonly used methodologies in developing the myocardial infarction model, the choice of animal species, the pre-operative antiarrhythmics prophylaxis, the choice of perioperative sedative, anaesthesia and analgesia, the immunosuppressive strategies in allowing xenotransplantation, the source of cells, number and delivery method are discussed.
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spelling pubmed-100507562023-03-30 Large animal models for cardiac remuscularization studies: A methodological review Yu, Yuexin Tham, Seng Kong Roslan, Fatin Fazrina Shaharuddin, Bakiah Yong, Yoke Keong Guo, Zhikun Tan, Jun Jie Front Cardiovasc Med Cardiovascular Medicine Myocardial infarction is the most common cause of heart failure, one of the most fatal non-communicable diseases worldwide. The disease could potentially be treated if the dead, ischemic heart tissues are regenerated and replaced with viable and functional cardiomyocytes. Pluripotent stem cells have proven the ability to derive specific and functional cardiomyocytes in large quantities for therapy. To test the remuscularization hypothesis, the strategy to model the disease in animals must resemble the pathophysiological conditions of myocardial infarction as in humans, to enable thorough testing of the safety and efficacy of the cardiomyocyte therapy before embarking on human trials. Rigorous experiments and in vivo findings using large mammals are increasingly important to simulate clinical reality and increase translatability into clinical practice. Hence, this review focus on large animal models which have been used in cardiac remuscularization studies using cardiomyocytes derived from human pluripotent stem cells. The commonly used methodologies in developing the myocardial infarction model, the choice of animal species, the pre-operative antiarrhythmics prophylaxis, the choice of perioperative sedative, anaesthesia and analgesia, the immunosuppressive strategies in allowing xenotransplantation, the source of cells, number and delivery method are discussed. Frontiers Media S.A. 2023-03-15 /pmc/articles/PMC10050756/ /pubmed/37008331 http://dx.doi.org/10.3389/fcvm.2023.1011880 Text en © 2023 Yu, Tham, Roslan, Shaharuddin, Yong, Guo and Tan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Yu, Yuexin
Tham, Seng Kong
Roslan, Fatin Fazrina
Shaharuddin, Bakiah
Yong, Yoke Keong
Guo, Zhikun
Tan, Jun Jie
Large animal models for cardiac remuscularization studies: A methodological review
title Large animal models for cardiac remuscularization studies: A methodological review
title_full Large animal models for cardiac remuscularization studies: A methodological review
title_fullStr Large animal models for cardiac remuscularization studies: A methodological review
title_full_unstemmed Large animal models for cardiac remuscularization studies: A methodological review
title_short Large animal models for cardiac remuscularization studies: A methodological review
title_sort large animal models for cardiac remuscularization studies: a methodological review
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050756/
https://www.ncbi.nlm.nih.gov/pubmed/37008331
http://dx.doi.org/10.3389/fcvm.2023.1011880
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