An optimized protocol to detect protein ubiquitination and activation by ubiquitination assay in vivo and CCK-8 assay

E3 ubiquitin ligases play a role in protein degradation, cellular localization, and activation, and their dysregulation is associated with human diseases. Here, we present a protocol to detect IGF2BP1 ubiquitination and activation by an E3 ubiquitin ligase FBXO45. We describe steps for preparing cel...

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Detalles Bibliográficos
Autores principales: Lin, Xiao-Tong, Zhang, Jie, Xie, Chuan-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050764/
https://www.ncbi.nlm.nih.gov/pubmed/36964909
http://dx.doi.org/10.1016/j.xpro.2023.102199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050764/
https://www.ncbi.nlm.nih.gov/pubmed/36964909
http://dx.doi.org/10.1016/j.xpro.2023.102199

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