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An optimized protocol to detect protein ubiquitination and activation by ubiquitination assay in vivo and CCK-8 assay
E3 ubiquitin ligases play a role in protein degradation, cellular localization, and activation, and their dysregulation is associated with human diseases. Here, we present a protocol to detect IGF2BP1 ubiquitination and activation by an E3 ubiquitin ligase FBXO45. We describe steps for preparing cel...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050764/ https://www.ncbi.nlm.nih.gov/pubmed/36964909 http://dx.doi.org/10.1016/j.xpro.2023.102199 |