Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerases

Here we present an optimized protocol for monitoring and analyzing single nucleotide incorporation by RNA polymerases. This protocol describes the assembly of Saccharomyces cerevisiae RNA polymerase I elongation complexes in a promoter-independent system in vitro. We describe how to collect a time c...

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Detalles Bibliográficos
Autores principales: Jacobs, Ruth Q., Bellis, Nathan F., Lucius, Aaron L., Schneider, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050783/
https://www.ncbi.nlm.nih.gov/pubmed/36964908
http://dx.doi.org/10.1016/j.xpro.2023.102191

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050783/
https://www.ncbi.nlm.nih.gov/pubmed/36964908
http://dx.doi.org/10.1016/j.xpro.2023.102191

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