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Mechanoenzymatic reactions for the hydrolysis of PET

Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer int...

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Autores principales: Ambrose-Dempster, Esther, Leipold, Leona, Dobrijevic, Dragana, Bawn, Maria, Carter, Eve M., Stojanovski, Gorjan, Sheppard, Tom D., Jeffries, Jack W. E., Ward, John M., Hailes, Helen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050947/
https://www.ncbi.nlm.nih.gov/pubmed/37006375
http://dx.doi.org/10.1039/d3ra01708g
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author Ambrose-Dempster, Esther
Leipold, Leona
Dobrijevic, Dragana
Bawn, Maria
Carter, Eve M.
Stojanovski, Gorjan
Sheppard, Tom D.
Jeffries, Jack W. E.
Ward, John M.
Hailes, Helen C.
author_facet Ambrose-Dempster, Esther
Leipold, Leona
Dobrijevic, Dragana
Bawn, Maria
Carter, Eve M.
Stojanovski, Gorjan
Sheppard, Tom D.
Jeffries, Jack W. E.
Ward, John M.
Hailes, Helen C.
author_sort Ambrose-Dempster, Esther
collection PubMed
description Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions.
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spelling pubmed-100509472023-03-30 Mechanoenzymatic reactions for the hydrolysis of PET Ambrose-Dempster, Esther Leipold, Leona Dobrijevic, Dragana Bawn, Maria Carter, Eve M. Stojanovski, Gorjan Sheppard, Tom D. Jeffries, Jack W. E. Ward, John M. Hailes, Helen C. RSC Adv Chemistry Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions. The Royal Society of Chemistry 2023-03-29 /pmc/articles/PMC10050947/ /pubmed/37006375 http://dx.doi.org/10.1039/d3ra01708g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Ambrose-Dempster, Esther
Leipold, Leona
Dobrijevic, Dragana
Bawn, Maria
Carter, Eve M.
Stojanovski, Gorjan
Sheppard, Tom D.
Jeffries, Jack W. E.
Ward, John M.
Hailes, Helen C.
Mechanoenzymatic reactions for the hydrolysis of PET
title Mechanoenzymatic reactions for the hydrolysis of PET
title_full Mechanoenzymatic reactions for the hydrolysis of PET
title_fullStr Mechanoenzymatic reactions for the hydrolysis of PET
title_full_unstemmed Mechanoenzymatic reactions for the hydrolysis of PET
title_short Mechanoenzymatic reactions for the hydrolysis of PET
title_sort mechanoenzymatic reactions for the hydrolysis of pet
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050947/
https://www.ncbi.nlm.nih.gov/pubmed/37006375
http://dx.doi.org/10.1039/d3ra01708g
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