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Mechanoenzymatic reactions for the hydrolysis of PET
Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer int...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050947/ https://www.ncbi.nlm.nih.gov/pubmed/37006375 http://dx.doi.org/10.1039/d3ra01708g |
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author | Ambrose-Dempster, Esther Leipold, Leona Dobrijevic, Dragana Bawn, Maria Carter, Eve M. Stojanovski, Gorjan Sheppard, Tom D. Jeffries, Jack W. E. Ward, John M. Hailes, Helen C. |
author_facet | Ambrose-Dempster, Esther Leipold, Leona Dobrijevic, Dragana Bawn, Maria Carter, Eve M. Stojanovski, Gorjan Sheppard, Tom D. Jeffries, Jack W. E. Ward, John M. Hailes, Helen C. |
author_sort | Ambrose-Dempster, Esther |
collection | PubMed |
description | Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions. |
format | Online Article Text |
id | pubmed-10050947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-100509472023-03-30 Mechanoenzymatic reactions for the hydrolysis of PET Ambrose-Dempster, Esther Leipold, Leona Dobrijevic, Dragana Bawn, Maria Carter, Eve M. Stojanovski, Gorjan Sheppard, Tom D. Jeffries, Jack W. E. Ward, John M. Hailes, Helen C. RSC Adv Chemistry Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions. The Royal Society of Chemistry 2023-03-29 /pmc/articles/PMC10050947/ /pubmed/37006375 http://dx.doi.org/10.1039/d3ra01708g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Ambrose-Dempster, Esther Leipold, Leona Dobrijevic, Dragana Bawn, Maria Carter, Eve M. Stojanovski, Gorjan Sheppard, Tom D. Jeffries, Jack W. E. Ward, John M. Hailes, Helen C. Mechanoenzymatic reactions for the hydrolysis of PET |
title | Mechanoenzymatic reactions for the hydrolysis of PET |
title_full | Mechanoenzymatic reactions for the hydrolysis of PET |
title_fullStr | Mechanoenzymatic reactions for the hydrolysis of PET |
title_full_unstemmed | Mechanoenzymatic reactions for the hydrolysis of PET |
title_short | Mechanoenzymatic reactions for the hydrolysis of PET |
title_sort | mechanoenzymatic reactions for the hydrolysis of pet |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10050947/ https://www.ncbi.nlm.nih.gov/pubmed/37006375 http://dx.doi.org/10.1039/d3ra01708g |
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