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Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model

Excisional wounds are considered one of the most common physical injuries. This study aims to test the effect of a nanophytosomal formulation loaded with a dried hydroalcoholic extract of S. platensis on promoting excisional wound healing. The Spirulina platensis nanophytosomal formulation (SPNP) co...

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Autores principales: Refai, Hanan, El-Gazar, Amira A., Ragab, Ghada M., Hassan, Doaa H., Ahmed, Omar S., Hussein, Rehab A., Shabana, Samah, Waffo-Téguo, Pierre, Valls, Josep, Al-Mokaddem, Asmaa K., Selim, Heba Mohammed Refat M., Yousef, Einas Mohamed, Ali, Sahar K., Salman, Ahmed, Abo-Zalam, Hagar B., Albash, Rofida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051127/
https://www.ncbi.nlm.nih.gov/pubmed/36976198
http://dx.doi.org/10.3390/md21030149
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author Refai, Hanan
El-Gazar, Amira A.
Ragab, Ghada M.
Hassan, Doaa H.
Ahmed, Omar S.
Hussein, Rehab A.
Shabana, Samah
Waffo-Téguo, Pierre
Valls, Josep
Al-Mokaddem, Asmaa K.
Selim, Heba Mohammed Refat M.
Yousef, Einas Mohamed
Ali, Sahar K.
Salman, Ahmed
Abo-Zalam, Hagar B.
Albash, Rofida
author_facet Refai, Hanan
El-Gazar, Amira A.
Ragab, Ghada M.
Hassan, Doaa H.
Ahmed, Omar S.
Hussein, Rehab A.
Shabana, Samah
Waffo-Téguo, Pierre
Valls, Josep
Al-Mokaddem, Asmaa K.
Selim, Heba Mohammed Refat M.
Yousef, Einas Mohamed
Ali, Sahar K.
Salman, Ahmed
Abo-Zalam, Hagar B.
Albash, Rofida
author_sort Refai, Hanan
collection PubMed
description Excisional wounds are considered one of the most common physical injuries. This study aims to test the effect of a nanophytosomal formulation loaded with a dried hydroalcoholic extract of S. platensis on promoting excisional wound healing. The Spirulina platensis nanophytosomal formulation (SPNP) containing 100 mg PC and 50 mg CH exhibited optimum physicochemical characteristics regarding particle size (598.40 ± 9.68 nm), zeta potential (−19.8 ± 0.49 mV), entrapment efficiency (62.76 ± 1.75%), and Q6h (74.00 ± 1.90%). It was selected to prepare an HPMC gel (SPNP-gel). Through metabolomic profiling of the algal extract, thirteen compounds were identified. Molecular docking of the identified compounds on the active site of the HMGB-1 protein revealed that 12,13-DiHome had the highest docking score of −7.130 kcal/mol. SPNP-gel showed higher wound closure potential and enhanced histopathological alterations as compared to standard (MEBO(®) ointment) and S. platensis gel in wounded Sprague-Dawley rats. Collectively, NPS promoted the wound healing process by enhancing the autophagy process (LC3B/Beclin-1) and the NRF-2/HO-1antioxidant pathway and halting the inflammatory (TNF-, NF-κB, TlR-4 and VEGF), apoptotic processes (AIF, Caspase-3), and the downregulation of HGMB-1 protein expression. The present study’s findings suggest that the topical application of SPNP-gel possesses a potential therapeutic effect in excisional wound healing, chiefly by downregulating HGMB-1 protein expression.
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spelling pubmed-100511272023-03-30 Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model Refai, Hanan El-Gazar, Amira A. Ragab, Ghada M. Hassan, Doaa H. Ahmed, Omar S. Hussein, Rehab A. Shabana, Samah Waffo-Téguo, Pierre Valls, Josep Al-Mokaddem, Asmaa K. Selim, Heba Mohammed Refat M. Yousef, Einas Mohamed Ali, Sahar K. Salman, Ahmed Abo-Zalam, Hagar B. Albash, Rofida Mar Drugs Article Excisional wounds are considered one of the most common physical injuries. This study aims to test the effect of a nanophytosomal formulation loaded with a dried hydroalcoholic extract of S. platensis on promoting excisional wound healing. The Spirulina platensis nanophytosomal formulation (SPNP) containing 100 mg PC and 50 mg CH exhibited optimum physicochemical characteristics regarding particle size (598.40 ± 9.68 nm), zeta potential (−19.8 ± 0.49 mV), entrapment efficiency (62.76 ± 1.75%), and Q6h (74.00 ± 1.90%). It was selected to prepare an HPMC gel (SPNP-gel). Through metabolomic profiling of the algal extract, thirteen compounds were identified. Molecular docking of the identified compounds on the active site of the HMGB-1 protein revealed that 12,13-DiHome had the highest docking score of −7.130 kcal/mol. SPNP-gel showed higher wound closure potential and enhanced histopathological alterations as compared to standard (MEBO(®) ointment) and S. platensis gel in wounded Sprague-Dawley rats. Collectively, NPS promoted the wound healing process by enhancing the autophagy process (LC3B/Beclin-1) and the NRF-2/HO-1antioxidant pathway and halting the inflammatory (TNF-, NF-κB, TlR-4 and VEGF), apoptotic processes (AIF, Caspase-3), and the downregulation of HGMB-1 protein expression. The present study’s findings suggest that the topical application of SPNP-gel possesses a potential therapeutic effect in excisional wound healing, chiefly by downregulating HGMB-1 protein expression. MDPI 2023-02-24 /pmc/articles/PMC10051127/ /pubmed/36976198 http://dx.doi.org/10.3390/md21030149 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Refai, Hanan
El-Gazar, Amira A.
Ragab, Ghada M.
Hassan, Doaa H.
Ahmed, Omar S.
Hussein, Rehab A.
Shabana, Samah
Waffo-Téguo, Pierre
Valls, Josep
Al-Mokaddem, Asmaa K.
Selim, Heba Mohammed Refat M.
Yousef, Einas Mohamed
Ali, Sahar K.
Salman, Ahmed
Abo-Zalam, Hagar B.
Albash, Rofida
Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title_full Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title_fullStr Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title_full_unstemmed Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title_short Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
title_sort enhanced wound healing potential of spirulina platensis nanophytosomes: metabolomic profiling, molecular networking, and modulation of hmgb-1 in an excisional wound rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051127/
https://www.ncbi.nlm.nih.gov/pubmed/36976198
http://dx.doi.org/10.3390/md21030149
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