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A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice

The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along wi...

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Autores principales: Alrouji, Mohammed, Kuriri, Fahd A., Alqasmi, Mohammed Hussein, AlSudais, Hamood, Alissa, Mohammed, Alsuwat, Meshari A., Asad, Mohammed, Joseph, Babu, Almuhanna, Yasir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051147/
https://www.ncbi.nlm.nih.gov/pubmed/36985266
http://dx.doi.org/10.3390/microorganisms11030692
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author Alrouji, Mohammed
Kuriri, Fahd A.
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
Almuhanna, Yasir
author_facet Alrouji, Mohammed
Kuriri, Fahd A.
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
Almuhanna, Yasir
author_sort Alrouji, Mohammed
collection PubMed
description The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy.
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spelling pubmed-100511472023-03-30 A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice Alrouji, Mohammed Kuriri, Fahd A. Alqasmi, Mohammed Hussein AlSudais, Hamood Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Almuhanna, Yasir Microorganisms Article The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy. MDPI 2023-03-08 /pmc/articles/PMC10051147/ /pubmed/36985266 http://dx.doi.org/10.3390/microorganisms11030692 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alrouji, Mohammed
Kuriri, Fahd A.
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
Almuhanna, Yasir
A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title_full A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title_fullStr A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title_full_unstemmed A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title_short A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
title_sort simple in-vivo method for evaluation of antibiofilm and wound healing activity using excision wound model in diabetic swiss albino mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051147/
https://www.ncbi.nlm.nih.gov/pubmed/36985266
http://dx.doi.org/10.3390/microorganisms11030692
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