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A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice
The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along wi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051147/ https://www.ncbi.nlm.nih.gov/pubmed/36985266 http://dx.doi.org/10.3390/microorganisms11030692 |
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author | Alrouji, Mohammed Kuriri, Fahd A. Alqasmi, Mohammed Hussein AlSudais, Hamood Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Almuhanna, Yasir |
author_facet | Alrouji, Mohammed Kuriri, Fahd A. Alqasmi, Mohammed Hussein AlSudais, Hamood Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Almuhanna, Yasir |
author_sort | Alrouji, Mohammed |
collection | PubMed |
description | The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy. |
format | Online Article Text |
id | pubmed-10051147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100511472023-03-30 A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice Alrouji, Mohammed Kuriri, Fahd A. Alqasmi, Mohammed Hussein AlSudais, Hamood Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Almuhanna, Yasir Microorganisms Article The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy. MDPI 2023-03-08 /pmc/articles/PMC10051147/ /pubmed/36985266 http://dx.doi.org/10.3390/microorganisms11030692 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alrouji, Mohammed Kuriri, Fahd A. Alqasmi, Mohammed Hussein AlSudais, Hamood Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Almuhanna, Yasir A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title | A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title_full | A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title_fullStr | A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title_full_unstemmed | A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title_short | A Simple In-Vivo Method for Evaluation of Antibiofilm and Wound Healing Activity Using Excision Wound Model in Diabetic Swiss Albino Mice |
title_sort | simple in-vivo method for evaluation of antibiofilm and wound healing activity using excision wound model in diabetic swiss albino mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051147/ https://www.ncbi.nlm.nih.gov/pubmed/36985266 http://dx.doi.org/10.3390/microorganisms11030692 |
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