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Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves
Interactions between programmed death 1 (PD-1) and PD-ligand 1 (PD-L1) cause functional exhaustion of T cells by inducing inhibitory signals, thereby attenuating effector functions of T cells. We have developed an anti-bovine PD-L1 blocking antibody (Ab) and have demonstrated that blockade of the in...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051159/ https://www.ncbi.nlm.nih.gov/pubmed/36992143 http://dx.doi.org/10.3390/vaccines11030559 |
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author | Okagawa, Tomohiro Konnai, Satoru Nakamura, Hayato Ganbaatar, Otgontuya Sajiki, Yamato Watari, Kei Noda, Haruka Honma, Mitsuru Kato, Yukinari Suzuki, Yasuhiko Maekawa, Naoya Murata, Shiro Ohashi, Kazuhiko |
author_facet | Okagawa, Tomohiro Konnai, Satoru Nakamura, Hayato Ganbaatar, Otgontuya Sajiki, Yamato Watari, Kei Noda, Haruka Honma, Mitsuru Kato, Yukinari Suzuki, Yasuhiko Maekawa, Naoya Murata, Shiro Ohashi, Kazuhiko |
author_sort | Okagawa, Tomohiro |
collection | PubMed |
description | Interactions between programmed death 1 (PD-1) and PD-ligand 1 (PD-L1) cause functional exhaustion of T cells by inducing inhibitory signals, thereby attenuating effector functions of T cells. We have developed an anti-bovine PD-L1 blocking antibody (Ab) and have demonstrated that blockade of the interaction between PD-1 and PD-L1 reactivates T-cell responses in cattle. In the present study, we examined the potential utility of PD-1/PD-L1-targeted immunotherapy in enhancing T-cell responses to vaccination. Calves were inoculated with a hexavalent live-attenuated viral vaccine against bovine respiratory infections in combination with treatment with an anti-PD-L1 Ab. The expression kinetics of PD-1 in T cells and T-cell responses to viral antigens were measured before and after vaccination to evaluate the adjuvant effect of anti-PD-L1 Ab. PD-1 expression was upregulated in vaccinated calves after the administration of a booster vaccination. The activation status of CD4(+), CD8(+), and γδTCR(+) T cells was enhanced by the combination of vaccination and PD-L1 blockade. In addition, IFN-γ responses to viral antigens were increased following combinatorial vaccination with PD-L1 blockade. In conclusion, the blockade of the PD-1/PD-L1 interaction enhances T-cell responses induced by vaccination in cattle, indicating the potential utility of anti-PD-L1 Ab in improving the efficacy of current vaccination programs. |
format | Online Article Text |
id | pubmed-10051159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100511592023-03-30 Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves Okagawa, Tomohiro Konnai, Satoru Nakamura, Hayato Ganbaatar, Otgontuya Sajiki, Yamato Watari, Kei Noda, Haruka Honma, Mitsuru Kato, Yukinari Suzuki, Yasuhiko Maekawa, Naoya Murata, Shiro Ohashi, Kazuhiko Vaccines (Basel) Article Interactions between programmed death 1 (PD-1) and PD-ligand 1 (PD-L1) cause functional exhaustion of T cells by inducing inhibitory signals, thereby attenuating effector functions of T cells. We have developed an anti-bovine PD-L1 blocking antibody (Ab) and have demonstrated that blockade of the interaction between PD-1 and PD-L1 reactivates T-cell responses in cattle. In the present study, we examined the potential utility of PD-1/PD-L1-targeted immunotherapy in enhancing T-cell responses to vaccination. Calves were inoculated with a hexavalent live-attenuated viral vaccine against bovine respiratory infections in combination with treatment with an anti-PD-L1 Ab. The expression kinetics of PD-1 in T cells and T-cell responses to viral antigens were measured before and after vaccination to evaluate the adjuvant effect of anti-PD-L1 Ab. PD-1 expression was upregulated in vaccinated calves after the administration of a booster vaccination. The activation status of CD4(+), CD8(+), and γδTCR(+) T cells was enhanced by the combination of vaccination and PD-L1 blockade. In addition, IFN-γ responses to viral antigens were increased following combinatorial vaccination with PD-L1 blockade. In conclusion, the blockade of the PD-1/PD-L1 interaction enhances T-cell responses induced by vaccination in cattle, indicating the potential utility of anti-PD-L1 Ab in improving the efficacy of current vaccination programs. MDPI 2023-03-01 /pmc/articles/PMC10051159/ /pubmed/36992143 http://dx.doi.org/10.3390/vaccines11030559 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Okagawa, Tomohiro Konnai, Satoru Nakamura, Hayato Ganbaatar, Otgontuya Sajiki, Yamato Watari, Kei Noda, Haruka Honma, Mitsuru Kato, Yukinari Suzuki, Yasuhiko Maekawa, Naoya Murata, Shiro Ohashi, Kazuhiko Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title_full | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title_fullStr | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title_full_unstemmed | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title_short | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
title_sort | enhancement of vaccine-induced t-cell responses by pd-l1 blockade in calves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051159/ https://www.ncbi.nlm.nih.gov/pubmed/36992143 http://dx.doi.org/10.3390/vaccines11030559 |
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