Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples

AIM: The current study investigated the performance of 4 widely used DNA extraction kits using different types of high (stool) and low biomass samples (chyme, broncho alveolar lavage and sputum). METHODS: Qiagen Powerfecal Pro DNA kit, Macherey Nucleospin Soil kit, Macherey Nucleospin Tissue Kit and...

Descripción completa

Detalles Bibliográficos
Autores principales: Gall-David, Sandrine Le, Boudry, Gaëlle, Buffet-Bataillon, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051199/
https://www.ncbi.nlm.nih.gov/pubmed/37006230
http://dx.doi.org/10.2144/fsoa-2022-0072
Descripción
Sumario:AIM: The current study investigated the performance of 4 widely used DNA extraction kits using different types of high (stool) and low biomass samples (chyme, broncho alveolar lavage and sputum). METHODS: Qiagen Powerfecal Pro DNA kit, Macherey Nucleospin Soil kit, Macherey Nucleospin Tissue Kit and MagnaPure LC DNA isolation kit III were evaluated in terms of DNA quantity, quality, diversity and composition profiles. RESULTS: The quantity and quality of DNA varied among the four kits. The microbiota of the stool samples showed similar diversity and compositional profiles for the 4 kits. CONCLUSION: Despite differences in DNA quality and quantity, the 4 kits yielded similar results for stool samples, while all kits were not sensitive enough for low biomass samples.

Ejemplares similares