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Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)

Pecan (Carya illinoensis) nuts are delicious and rich in unsaturated fatty acids, which are beneficial for human health. Their yield is closely related to several factors, such as the ratio of female and male flowers. We sampled and paraffin-sectioned female and male flower buds for one year and det...

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Autores principales: Xie, Yifei, Hou, Zhiying, Shi, Miao, Wang, Qiaoyan, Yang, Zhengfu, Lim, Kean-Jin, Wang, Zhengjia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051282/
https://www.ncbi.nlm.nih.gov/pubmed/36987065
http://dx.doi.org/10.3390/plants12061378
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author Xie, Yifei
Hou, Zhiying
Shi, Miao
Wang, Qiaoyan
Yang, Zhengfu
Lim, Kean-Jin
Wang, Zhengjia
author_facet Xie, Yifei
Hou, Zhiying
Shi, Miao
Wang, Qiaoyan
Yang, Zhengfu
Lim, Kean-Jin
Wang, Zhengjia
author_sort Xie, Yifei
collection PubMed
description Pecan (Carya illinoensis) nuts are delicious and rich in unsaturated fatty acids, which are beneficial for human health. Their yield is closely related to several factors, such as the ratio of female and male flowers. We sampled and paraffin-sectioned female and male flower buds for one year and determined the stages of initial flower bud differentiation, floral primordium formation, and pistil and stamen primordium formation. We then performed transcriptome sequencing on these stages. Our data analysis suggested that FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 play a role in flower bud differentiation. J3 was highly expressed in the early stage of female flower buds and may play a role in regulating flower bud differentiation and flowering time. Genes such as NF-YA1 and STM were expressed during male flower bud development. NF-YA1 belongs to the NF-Y transcription factor family and may initiate downstream events leading to floral transformation. STM promoted the transformation of leaf buds to flower buds. AP2 may have been involved in the establishment of floral meristem characteristics and the determination of floral organ characteristics. Our results lay a foundation for the control and subsequent regulation of female and male flower bud differentiation and yield improvement.
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spelling pubmed-100512822023-03-30 Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis) Xie, Yifei Hou, Zhiying Shi, Miao Wang, Qiaoyan Yang, Zhengfu Lim, Kean-Jin Wang, Zhengjia Plants (Basel) Article Pecan (Carya illinoensis) nuts are delicious and rich in unsaturated fatty acids, which are beneficial for human health. Their yield is closely related to several factors, such as the ratio of female and male flowers. We sampled and paraffin-sectioned female and male flower buds for one year and determined the stages of initial flower bud differentiation, floral primordium formation, and pistil and stamen primordium formation. We then performed transcriptome sequencing on these stages. Our data analysis suggested that FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 play a role in flower bud differentiation. J3 was highly expressed in the early stage of female flower buds and may play a role in regulating flower bud differentiation and flowering time. Genes such as NF-YA1 and STM were expressed during male flower bud development. NF-YA1 belongs to the NF-Y transcription factor family and may initiate downstream events leading to floral transformation. STM promoted the transformation of leaf buds to flower buds. AP2 may have been involved in the establishment of floral meristem characteristics and the determination of floral organ characteristics. Our results lay a foundation for the control and subsequent regulation of female and male flower bud differentiation and yield improvement. MDPI 2023-03-20 /pmc/articles/PMC10051282/ /pubmed/36987065 http://dx.doi.org/10.3390/plants12061378 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Yifei
Hou, Zhiying
Shi, Miao
Wang, Qiaoyan
Yang, Zhengfu
Lim, Kean-Jin
Wang, Zhengjia
Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title_full Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title_fullStr Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title_full_unstemmed Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title_short Transcriptional Regulation of Female and Male Flower Bud Initiation and Development in Pecan (Carya illinoensis)
title_sort transcriptional regulation of female and male flower bud initiation and development in pecan (carya illinoensis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051282/
https://www.ncbi.nlm.nih.gov/pubmed/36987065
http://dx.doi.org/10.3390/plants12061378
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