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Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)

During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pube...

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Autores principales: Benchimol, Gabriela da Costa, Santos, Josye Bianca, Lopes, Ana Sophia da Costa, Oliveira, Karol Guimarães, Okada, Eviny Sayuri Trindade, de Alcantara, Bianca Nascimento, Pereira, Washington Luiz Assunção, Leão, Danuza Leite, Martins, Ana Cristina Carneiro, Carneiro, Liliane Almeida, Imbeloni, Aline Amaral, Makiama, Sheila Tetsume, de Castro, Luiz Paulo Printes Albarelli, Coutinho, Leandro Nassar, Casseb, Lívia Medeiros Neves, Vasconcelos, Pedro Fernando da Costa, Domingues, Sheyla Farhayldes Souza, Medeiros, Daniele Barbosa de Almeida, Scalercio, Sarah Raphaella Rocha de Azevedo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051343/
https://www.ncbi.nlm.nih.gov/pubmed/36992324
http://dx.doi.org/10.3390/v15030615
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author Benchimol, Gabriela da Costa
Santos, Josye Bianca
Lopes, Ana Sophia da Costa
Oliveira, Karol Guimarães
Okada, Eviny Sayuri Trindade
de Alcantara, Bianca Nascimento
Pereira, Washington Luiz Assunção
Leão, Danuza Leite
Martins, Ana Cristina Carneiro
Carneiro, Liliane Almeida
Imbeloni, Aline Amaral
Makiama, Sheila Tetsume
de Castro, Luiz Paulo Printes Albarelli
Coutinho, Leandro Nassar
Casseb, Lívia Medeiros Neves
Vasconcelos, Pedro Fernando da Costa
Domingues, Sheyla Farhayldes Souza
Medeiros, Daniele Barbosa de Almeida
Scalercio, Sarah Raphaella Rocha de Azevedo
author_facet Benchimol, Gabriela da Costa
Santos, Josye Bianca
Lopes, Ana Sophia da Costa
Oliveira, Karol Guimarães
Okada, Eviny Sayuri Trindade
de Alcantara, Bianca Nascimento
Pereira, Washington Luiz Assunção
Leão, Danuza Leite
Martins, Ana Cristina Carneiro
Carneiro, Liliane Almeida
Imbeloni, Aline Amaral
Makiama, Sheila Tetsume
de Castro, Luiz Paulo Printes Albarelli
Coutinho, Leandro Nassar
Casseb, Lívia Medeiros Neves
Vasconcelos, Pedro Fernando da Costa
Domingues, Sheyla Farhayldes Souza
Medeiros, Daniele Barbosa de Almeida
Scalercio, Sarah Raphaella Rocha de Azevedo
author_sort Benchimol, Gabriela da Costa
collection PubMed
description During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pubertal squirrel monkeys (Saimiri collinsi) infected with ZIKV, analyzing the effects at different stages of infection. The susceptibility of S. collinsi to ZIKV infection was confirmed by laboratory tests, which detected viremia (mean 1.63 × 10(6) RNA copies/µL) and IgM antibody induction. Reduced fecal testosterone levels, severe testicular atrophy and prolonged orchitis were observed throughout the experiment by ultrasound. At 21 dpi, testicular damage associated with ZIKV was confirmed by histopathological and immunohistochemical (IHC) analyses. Tubular retraction, the degeneration and necrosis of somatic and germ cells in the seminiferous tubules, the proliferation of interstitial cells and an inflammatory infiltrate were observed. ZIKV antigen was identified in the same cells where tissue injuries were observed. In conclusion, squirrel monkeys were found to be susceptible to the Asian variant of ZIKV, and this model enabled the identification of multifocal lesions in the seminiferous tubules of the infected group evaluated. These findings may suggest an impact of ZIKV infection on male fertility.
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spelling pubmed-100513432023-03-30 Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi) Benchimol, Gabriela da Costa Santos, Josye Bianca Lopes, Ana Sophia da Costa Oliveira, Karol Guimarães Okada, Eviny Sayuri Trindade de Alcantara, Bianca Nascimento Pereira, Washington Luiz Assunção Leão, Danuza Leite Martins, Ana Cristina Carneiro Carneiro, Liliane Almeida Imbeloni, Aline Amaral Makiama, Sheila Tetsume de Castro, Luiz Paulo Printes Albarelli Coutinho, Leandro Nassar Casseb, Lívia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Domingues, Sheyla Farhayldes Souza Medeiros, Daniele Barbosa de Almeida Scalercio, Sarah Raphaella Rocha de Azevedo Viruses Article During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pubertal squirrel monkeys (Saimiri collinsi) infected with ZIKV, analyzing the effects at different stages of infection. The susceptibility of S. collinsi to ZIKV infection was confirmed by laboratory tests, which detected viremia (mean 1.63 × 10(6) RNA copies/µL) and IgM antibody induction. Reduced fecal testosterone levels, severe testicular atrophy and prolonged orchitis were observed throughout the experiment by ultrasound. At 21 dpi, testicular damage associated with ZIKV was confirmed by histopathological and immunohistochemical (IHC) analyses. Tubular retraction, the degeneration and necrosis of somatic and germ cells in the seminiferous tubules, the proliferation of interstitial cells and an inflammatory infiltrate were observed. ZIKV antigen was identified in the same cells where tissue injuries were observed. In conclusion, squirrel monkeys were found to be susceptible to the Asian variant of ZIKV, and this model enabled the identification of multifocal lesions in the seminiferous tubules of the infected group evaluated. These findings may suggest an impact of ZIKV infection on male fertility. MDPI 2023-02-23 /pmc/articles/PMC10051343/ /pubmed/36992324 http://dx.doi.org/10.3390/v15030615 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benchimol, Gabriela da Costa
Santos, Josye Bianca
Lopes, Ana Sophia da Costa
Oliveira, Karol Guimarães
Okada, Eviny Sayuri Trindade
de Alcantara, Bianca Nascimento
Pereira, Washington Luiz Assunção
Leão, Danuza Leite
Martins, Ana Cristina Carneiro
Carneiro, Liliane Almeida
Imbeloni, Aline Amaral
Makiama, Sheila Tetsume
de Castro, Luiz Paulo Printes Albarelli
Coutinho, Leandro Nassar
Casseb, Lívia Medeiros Neves
Vasconcelos, Pedro Fernando da Costa
Domingues, Sheyla Farhayldes Souza
Medeiros, Daniele Barbosa de Almeida
Scalercio, Sarah Raphaella Rocha de Azevedo
Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title_full Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title_fullStr Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title_full_unstemmed Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title_short Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)
title_sort zika virus infection damages the testes in pubertal common squirrel monkeys (saimiri collinsi)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051343/
https://www.ncbi.nlm.nih.gov/pubmed/36992324
http://dx.doi.org/10.3390/v15030615
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