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Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus

Progesterone functions as a steroid hormone involved in female reproductive physiology. While some reproductive disorders manifest with symptoms that can be treated by progesterone or synthetic progestins, recent data suggest that women also seek botanical supplements to alleviate these symptoms. Ho...

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Autores principales: Bergsten, Tova M., Li, Kailiang, Lantvit, Daniel D., Murphy, Brian T., Burdette, Joanna E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051346/
https://www.ncbi.nlm.nih.gov/pubmed/36986136
http://dx.doi.org/10.3390/nu15061407
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author Bergsten, Tova M.
Li, Kailiang
Lantvit, Daniel D.
Murphy, Brian T.
Burdette, Joanna E.
author_facet Bergsten, Tova M.
Li, Kailiang
Lantvit, Daniel D.
Murphy, Brian T.
Burdette, Joanna E.
author_sort Bergsten, Tova M.
collection PubMed
description Progesterone functions as a steroid hormone involved in female reproductive physiology. While some reproductive disorders manifest with symptoms that can be treated by progesterone or synthetic progestins, recent data suggest that women also seek botanical supplements to alleviate these symptoms. However, botanical supplements are not regulated by the U.S. Food and Drug Administration and therefore it is important to characterize and quantify the inherent active compounds and biological targets of supplements within cellular and animal systems. In this study, we analyzed the effect of two natural products, the flavonoids, apigenin and kaempferol, to determine their relationship to progesterone treatment in vivo. According to immunohistochemical analysis of uterine tissue, kaempferol and apigenin have some progestogenic activity, but do not act in exactly the same manner as progesterone. More specifically, kaempferol treatment did not induce HAND2, did not change proliferation, and induced ZBTB16 expression. Additionally, while apigenin treatment did not appear to dramatically affect transcripts, kaempferol treatment altered some transcripts (44%) in a similar manner to progesterone treatment but had some unique effects as well. Kaempferol regulated primarily unfolded protein response, androgen response, and interferon-related transcripts in a similar manner to progesterone. However, the effects of progesterone were more significant in regulating thousands of transcripts making kaempferol a selective modifier of signaling in the mouse uterus. In summary, the phytoprogestins, apigenin and kaempferol, have progestogenic activity in vivo but also act uniquely.
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spelling pubmed-100513462023-03-30 Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus Bergsten, Tova M. Li, Kailiang Lantvit, Daniel D. Murphy, Brian T. Burdette, Joanna E. Nutrients Article Progesterone functions as a steroid hormone involved in female reproductive physiology. While some reproductive disorders manifest with symptoms that can be treated by progesterone or synthetic progestins, recent data suggest that women also seek botanical supplements to alleviate these symptoms. However, botanical supplements are not regulated by the U.S. Food and Drug Administration and therefore it is important to characterize and quantify the inherent active compounds and biological targets of supplements within cellular and animal systems. In this study, we analyzed the effect of two natural products, the flavonoids, apigenin and kaempferol, to determine their relationship to progesterone treatment in vivo. According to immunohistochemical analysis of uterine tissue, kaempferol and apigenin have some progestogenic activity, but do not act in exactly the same manner as progesterone. More specifically, kaempferol treatment did not induce HAND2, did not change proliferation, and induced ZBTB16 expression. Additionally, while apigenin treatment did not appear to dramatically affect transcripts, kaempferol treatment altered some transcripts (44%) in a similar manner to progesterone treatment but had some unique effects as well. Kaempferol regulated primarily unfolded protein response, androgen response, and interferon-related transcripts in a similar manner to progesterone. However, the effects of progesterone were more significant in regulating thousands of transcripts making kaempferol a selective modifier of signaling in the mouse uterus. In summary, the phytoprogestins, apigenin and kaempferol, have progestogenic activity in vivo but also act uniquely. MDPI 2023-03-15 /pmc/articles/PMC10051346/ /pubmed/36986136 http://dx.doi.org/10.3390/nu15061407 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bergsten, Tova M.
Li, Kailiang
Lantvit, Daniel D.
Murphy, Brian T.
Burdette, Joanna E.
Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title_full Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title_fullStr Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title_full_unstemmed Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title_short Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus
title_sort kaempferol, a phytoprogestin, induces a subset of progesterone-regulated genes in the uterus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051346/
https://www.ncbi.nlm.nih.gov/pubmed/36986136
http://dx.doi.org/10.3390/nu15061407
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