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Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode
Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modifi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051639/ https://www.ncbi.nlm.nih.gov/pubmed/36991919 http://dx.doi.org/10.3390/s23063209 |
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author | Li, Yang-Yang Ma, Xin-Xin Song, Xin-Yan Ma, Lin-Lin Li, Yu-Ying Meng, Xin Chen, Yu-Jie Xu, Ke-Xin Moosavi-Movahedi, Ali Akbar Xiao, Bao-Lin Hong, Jun |
author_facet | Li, Yang-Yang Ma, Xin-Xin Song, Xin-Yan Ma, Lin-Lin Li, Yu-Ying Meng, Xin Chen, Yu-Jie Xu, Ke-Xin Moosavi-Movahedi, Ali Akbar Xiao, Bao-Lin Hong, Jun |
author_sort | Li, Yang-Yang |
collection | PubMed |
description | Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM(−1)·cm(−2)), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constant [Formula: see text] is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory. |
format | Online Article Text |
id | pubmed-10051639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100516392023-03-30 Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode Li, Yang-Yang Ma, Xin-Xin Song, Xin-Yan Ma, Lin-Lin Li, Yu-Ying Meng, Xin Chen, Yu-Jie Xu, Ke-Xin Moosavi-Movahedi, Ali Akbar Xiao, Bao-Lin Hong, Jun Sensors (Basel) Article Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM(−1)·cm(−2)), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constant [Formula: see text] is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory. MDPI 2023-03-17 /pmc/articles/PMC10051639/ /pubmed/36991919 http://dx.doi.org/10.3390/s23063209 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Yang-Yang Ma, Xin-Xin Song, Xin-Yan Ma, Lin-Lin Li, Yu-Ying Meng, Xin Chen, Yu-Jie Xu, Ke-Xin Moosavi-Movahedi, Ali Akbar Xiao, Bao-Lin Hong, Jun Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title_full | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title_fullStr | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title_full_unstemmed | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title_short | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
title_sort | glucose biosensor based on glucose oxidase immobilized on bsa cross-linked nanocomposite modified glassy carbon electrode |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051639/ https://www.ncbi.nlm.nih.gov/pubmed/36991919 http://dx.doi.org/10.3390/s23063209 |
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