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Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei

The Pacific white shrimp Litopenaeus vannamei is the most economically important crustacean in the world. The growth and development of shrimp muscle has always been the focus of attention. Myocyte Enhancer Factor 2 (MEF2), a member of MADS transcription factor, has an essential influence on various...

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Autores principales: Xia, Yanting, Zhong, Xiaoyun, Zhang, Xiaoxi, Zhang, Xiaojun, Yuan, Jianbo, Liu, Chengzhang, Sha, Zhenxia, Li, Fuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051702/
https://www.ncbi.nlm.nih.gov/pubmed/36982906
http://dx.doi.org/10.3390/ijms24065832
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author Xia, Yanting
Zhong, Xiaoyun
Zhang, Xiaoxi
Zhang, Xiaojun
Yuan, Jianbo
Liu, Chengzhang
Sha, Zhenxia
Li, Fuhua
author_facet Xia, Yanting
Zhong, Xiaoyun
Zhang, Xiaoxi
Zhang, Xiaojun
Yuan, Jianbo
Liu, Chengzhang
Sha, Zhenxia
Li, Fuhua
author_sort Xia, Yanting
collection PubMed
description The Pacific white shrimp Litopenaeus vannamei is the most economically important crustacean in the world. The growth and development of shrimp muscle has always been the focus of attention. Myocyte Enhancer Factor 2 (MEF2), a member of MADS transcription factor, has an essential influence on various growth and development programs, including myogenesis. In this study, based on the genome and transcriptome data of L. vannamei, the gene structure and expression profiles of MEF2 were characterized. We found that the LvMEF2 was widely expressed in various tissues, mainly in the Oka organ, brain, intestine, heart, and muscle. Moreover, LvMEF2 has a large number of splice variants, and the main forms are the mutually exclusive exon and alternative 5′ splice site. The expression profiles of the LvMEF2 splice variants varied under different conditions. Interestingly, some splice variants have tissue or developmental expression specificity. After RNA interference into LvMEF2, the increment in the body length and weight decreased significantly and even caused death, suggesting that LvMEF2 can affect the growth and survival of L. vannamei. Transcriptome analysis showed that after LvMEF2 was knocked down, the protein synthesis and immune-related pathways were affected, and the associated muscle protein synthesis decreased, indicating that LvMEF2 affected muscle formation and the immune system. The results provide an important basis for future studies of the MEF2 gene and the mechanism of muscle growth and development in shrimp.
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spelling pubmed-100517022023-03-30 Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei Xia, Yanting Zhong, Xiaoyun Zhang, Xiaoxi Zhang, Xiaojun Yuan, Jianbo Liu, Chengzhang Sha, Zhenxia Li, Fuhua Int J Mol Sci Article The Pacific white shrimp Litopenaeus vannamei is the most economically important crustacean in the world. The growth and development of shrimp muscle has always been the focus of attention. Myocyte Enhancer Factor 2 (MEF2), a member of MADS transcription factor, has an essential influence on various growth and development programs, including myogenesis. In this study, based on the genome and transcriptome data of L. vannamei, the gene structure and expression profiles of MEF2 were characterized. We found that the LvMEF2 was widely expressed in various tissues, mainly in the Oka organ, brain, intestine, heart, and muscle. Moreover, LvMEF2 has a large number of splice variants, and the main forms are the mutually exclusive exon and alternative 5′ splice site. The expression profiles of the LvMEF2 splice variants varied under different conditions. Interestingly, some splice variants have tissue or developmental expression specificity. After RNA interference into LvMEF2, the increment in the body length and weight decreased significantly and even caused death, suggesting that LvMEF2 can affect the growth and survival of L. vannamei. Transcriptome analysis showed that after LvMEF2 was knocked down, the protein synthesis and immune-related pathways were affected, and the associated muscle protein synthesis decreased, indicating that LvMEF2 affected muscle formation and the immune system. The results provide an important basis for future studies of the MEF2 gene and the mechanism of muscle growth and development in shrimp. MDPI 2023-03-18 /pmc/articles/PMC10051702/ /pubmed/36982906 http://dx.doi.org/10.3390/ijms24065832 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xia, Yanting
Zhong, Xiaoyun
Zhang, Xiaoxi
Zhang, Xiaojun
Yuan, Jianbo
Liu, Chengzhang
Sha, Zhenxia
Li, Fuhua
Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title_full Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title_fullStr Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title_full_unstemmed Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title_short Gene Structure, Expression and Function Analysis of MEF2 in the Pacific White Shrimp Litopenaeus vannamei
title_sort gene structure, expression and function analysis of mef2 in the pacific white shrimp litopenaeus vannamei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051702/
https://www.ncbi.nlm.nih.gov/pubmed/36982906
http://dx.doi.org/10.3390/ijms24065832
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