Cargando…
Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro
Phytomonas serpens is a trypanosomatid phytoparasite, found in a great variety of species, including tomato plants. It is a significant problem for agriculture, causing high economic loss. In order to reduce the vegetal infections, different strategies have been used. The biological activity of mole...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051746/ https://www.ncbi.nlm.nih.gov/pubmed/36986345 http://dx.doi.org/10.3390/pathogens12030423 |
_version_ | 1785014963734052864 |
---|---|
author | Santos, Tamiris A. C. Silva, Kleiton P. Souza, Gabriella B. Alves, Péricles B. Menna-Barreto, Rubem F. S. Scher, Ricardo Fernandes, Roberta P. M. |
author_facet | Santos, Tamiris A. C. Silva, Kleiton P. Souza, Gabriella B. Alves, Péricles B. Menna-Barreto, Rubem F. S. Scher, Ricardo Fernandes, Roberta P. M. |
author_sort | Santos, Tamiris A. C. |
collection | PubMed |
description | Phytomonas serpens is a trypanosomatid phytoparasite, found in a great variety of species, including tomato plants. It is a significant problem for agriculture, causing high economic loss. In order to reduce the vegetal infections, different strategies have been used. The biological activity of molecules obtained from natural sources has been widely investigated to treat trypanosomatids infections. Among these compounds, chalcones have been shown to have anti-parasitic and anti-inflammatory effects, being described as having a remarkable activity on trypanosomatids, especially in Leishmania species. Here, we evaluated the antiprotozoal activity of the chalcone derivative (NaF) on P. serpens promastigotes, while also assessing its mechanism of action. The results showed that treatment with the derivative NaF for 24 h promotes an important reduction in the parasite proliferation (IC(50)/24 h = 23.6 ± 4.6 µM). At IC(50)/24 h concentration, the compound induced an increase in reactive oxygen species (ROS) production and a shortening of the unique flagellum of the parasites. Electron microscopy evaluation reinforced the flagellar phenotype in treated promastigotes, and a dilated flagellar pocket was frequently observed. The treatment also promoted a prominent autophagic phenotype. An increased number of autophagosomes were detected, presenting different levels of cargo degradation, endoplasmic reticulum profiles surrounding different cellular structures, and the presence of concentric membranar structures inside the mitochondrion. Chalcone derivatives may present an opportunity to develop a treatment for the P. serpens infection, as they are easy to synthesize and are low in cost. In order to develop a new product, further studies are still necessary. |
format | Online Article Text |
id | pubmed-10051746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100517462023-03-30 Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro Santos, Tamiris A. C. Silva, Kleiton P. Souza, Gabriella B. Alves, Péricles B. Menna-Barreto, Rubem F. S. Scher, Ricardo Fernandes, Roberta P. M. Pathogens Article Phytomonas serpens is a trypanosomatid phytoparasite, found in a great variety of species, including tomato plants. It is a significant problem for agriculture, causing high economic loss. In order to reduce the vegetal infections, different strategies have been used. The biological activity of molecules obtained from natural sources has been widely investigated to treat trypanosomatids infections. Among these compounds, chalcones have been shown to have anti-parasitic and anti-inflammatory effects, being described as having a remarkable activity on trypanosomatids, especially in Leishmania species. Here, we evaluated the antiprotozoal activity of the chalcone derivative (NaF) on P. serpens promastigotes, while also assessing its mechanism of action. The results showed that treatment with the derivative NaF for 24 h promotes an important reduction in the parasite proliferation (IC(50)/24 h = 23.6 ± 4.6 µM). At IC(50)/24 h concentration, the compound induced an increase in reactive oxygen species (ROS) production and a shortening of the unique flagellum of the parasites. Electron microscopy evaluation reinforced the flagellar phenotype in treated promastigotes, and a dilated flagellar pocket was frequently observed. The treatment also promoted a prominent autophagic phenotype. An increased number of autophagosomes were detected, presenting different levels of cargo degradation, endoplasmic reticulum profiles surrounding different cellular structures, and the presence of concentric membranar structures inside the mitochondrion. Chalcone derivatives may present an opportunity to develop a treatment for the P. serpens infection, as they are easy to synthesize and are low in cost. In order to develop a new product, further studies are still necessary. MDPI 2023-03-07 /pmc/articles/PMC10051746/ /pubmed/36986345 http://dx.doi.org/10.3390/pathogens12030423 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santos, Tamiris A. C. Silva, Kleiton P. Souza, Gabriella B. Alves, Péricles B. Menna-Barreto, Rubem F. S. Scher, Ricardo Fernandes, Roberta P. M. Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title | Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title_full | Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title_fullStr | Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title_full_unstemmed | Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title_short | Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro |
title_sort | chalcone derivative induces flagellar disruption and autophagic phenotype in phytomonas serpens in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051746/ https://www.ncbi.nlm.nih.gov/pubmed/36986345 http://dx.doi.org/10.3390/pathogens12030423 |
work_keys_str_mv | AT santostamirisac chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT silvakleitonp chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT souzagabriellab chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT alvespericlesb chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT mennabarretorubemfs chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT scherricardo chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro AT fernandesrobertapm chalconederivativeinducesflagellardisruptionandautophagicphenotypeinphytomonasserpensinvitro |