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Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography

Aflatoxins (AFs) are a group of secondary metabolites that cause several diseases in both animals and humans. Since the discovery of this group of toxins, several effects were revealed, such as hepatic changes, carcinoma, failure, and cancer of the liver. In the European Union, there are concentrati...

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Autores principales: Endre, Gábor, Nagy, Babett Edit, Hercegfalvi, Dániel, Kasuba, Csenge, Vágvölgyi, Csaba, Szekeres, András
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051833/
https://www.ncbi.nlm.nih.gov/pubmed/36977068
http://dx.doi.org/10.3390/toxins15030178
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author Endre, Gábor
Nagy, Babett Edit
Hercegfalvi, Dániel
Kasuba, Csenge
Vágvölgyi, Csaba
Szekeres, András
author_facet Endre, Gábor
Nagy, Babett Edit
Hercegfalvi, Dániel
Kasuba, Csenge
Vágvölgyi, Csaba
Szekeres, András
author_sort Endre, Gábor
collection PubMed
description Aflatoxins (AFs) are a group of secondary metabolites that cause several diseases in both animals and humans. Since the discovery of this group of toxins, several effects were revealed, such as hepatic changes, carcinoma, failure, and cancer of the liver. In the European Union, there are concentration limits for this group of mycotoxins in food and feed products; thus, these substances are required in their pure forms to prepare reference standards or certified reference materials. In our present work, a liquid–liquid chromatographic method utilizing a toluene/acetic acid/water ternary system was improved. In order to enhance the purification and gain a higher amount of pure AFs in one separation run, a scale-up of the previous separation was carried out. In several scale-up steps—including the determination of the maximum concentration and volume to load on a 250 mL rotor via a loop and via a pump as well, and the quadruplication of the entire separation procedure to a 1000 mL rotor—an efficient scale-up was achieved. Utilizing a 250 mL rotor in an 8-hour workday, altogether approximately 2.2 g of total AFs could be purified with 8.2 liters of solvent, while on a 1000 mL column, approximately 7.8 g AFs could be prepared, utilizing around 31 liters of solvents.
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spelling pubmed-100518332023-03-30 Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography Endre, Gábor Nagy, Babett Edit Hercegfalvi, Dániel Kasuba, Csenge Vágvölgyi, Csaba Szekeres, András Toxins (Basel) Communication Aflatoxins (AFs) are a group of secondary metabolites that cause several diseases in both animals and humans. Since the discovery of this group of toxins, several effects were revealed, such as hepatic changes, carcinoma, failure, and cancer of the liver. In the European Union, there are concentration limits for this group of mycotoxins in food and feed products; thus, these substances are required in their pure forms to prepare reference standards or certified reference materials. In our present work, a liquid–liquid chromatographic method utilizing a toluene/acetic acid/water ternary system was improved. In order to enhance the purification and gain a higher amount of pure AFs in one separation run, a scale-up of the previous separation was carried out. In several scale-up steps—including the determination of the maximum concentration and volume to load on a 250 mL rotor via a loop and via a pump as well, and the quadruplication of the entire separation procedure to a 1000 mL rotor—an efficient scale-up was achieved. Utilizing a 250 mL rotor in an 8-hour workday, altogether approximately 2.2 g of total AFs could be purified with 8.2 liters of solvent, while on a 1000 mL column, approximately 7.8 g AFs could be prepared, utilizing around 31 liters of solvents. MDPI 2023-02-25 /pmc/articles/PMC10051833/ /pubmed/36977068 http://dx.doi.org/10.3390/toxins15030178 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Endre, Gábor
Nagy, Babett Edit
Hercegfalvi, Dániel
Kasuba, Csenge
Vágvölgyi, Csaba
Szekeres, András
Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title_full Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title_fullStr Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title_full_unstemmed Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title_short Scale-up of Aflatoxin Purification by Centrifugal Partition Chromatography
title_sort scale-up of aflatoxin purification by centrifugal partition chromatography
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051833/
https://www.ncbi.nlm.nih.gov/pubmed/36977068
http://dx.doi.org/10.3390/toxins15030178
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