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Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine

Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fet...

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Autores principales: Matsui, Kenji, Kinoshita, Yoshitaka, Inage, Yuka, Matsumoto, Naoto, Morimoto, Keita, Saito, Yatsumu, Takamura, Tsuyoshi, Matsunari, Hitomi, Yamanaka, Shuichiro, Nagashima, Hiroshi, Kobayashi, Eiji, Yokoo, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051840/
https://www.ncbi.nlm.nih.gov/pubmed/36983293
http://dx.doi.org/10.3390/jcm12062293
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author Matsui, Kenji
Kinoshita, Yoshitaka
Inage, Yuka
Matsumoto, Naoto
Morimoto, Keita
Saito, Yatsumu
Takamura, Tsuyoshi
Matsunari, Hitomi
Yamanaka, Shuichiro
Nagashima, Hiroshi
Kobayashi, Eiji
Yokoo, Takashi
author_facet Matsui, Kenji
Kinoshita, Yoshitaka
Inage, Yuka
Matsumoto, Naoto
Morimoto, Keita
Saito, Yatsumu
Takamura, Tsuyoshi
Matsunari, Hitomi
Yamanaka, Shuichiro
Nagashima, Hiroshi
Kobayashi, Eiji
Yokoo, Takashi
author_sort Matsui, Kenji
collection PubMed
description Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with human nephron progenitor cells. For clinical application, it is desirable to establish reliable methods for the preservation and quality assessment of grafts. We evaluated the differentiation potency of vitrified porcine fetal kidneys compared with nonfrozen kidneys, using an in vivo differentiation model. Fetal porcine kidneys connected to the bladder were frozen via vitrification and stored in liquid nitrogen. Several days later, they were thawed and transplanted under the retroperitoneum of immunocompromised mice. After 14 days, the frozen kidneys grew and differentiated into mature nephrons, and the findings were comparable to those of nonfrozen kidneys. In conclusion, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated using this model, thereby providing a practical protocol to assess the quality of individual lots.
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spelling pubmed-100518402023-03-30 Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine Matsui, Kenji Kinoshita, Yoshitaka Inage, Yuka Matsumoto, Naoto Morimoto, Keita Saito, Yatsumu Takamura, Tsuyoshi Matsunari, Hitomi Yamanaka, Shuichiro Nagashima, Hiroshi Kobayashi, Eiji Yokoo, Takashi J Clin Med Brief Report Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with human nephron progenitor cells. For clinical application, it is desirable to establish reliable methods for the preservation and quality assessment of grafts. We evaluated the differentiation potency of vitrified porcine fetal kidneys compared with nonfrozen kidneys, using an in vivo differentiation model. Fetal porcine kidneys connected to the bladder were frozen via vitrification and stored in liquid nitrogen. Several days later, they were thawed and transplanted under the retroperitoneum of immunocompromised mice. After 14 days, the frozen kidneys grew and differentiated into mature nephrons, and the findings were comparable to those of nonfrozen kidneys. In conclusion, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated using this model, thereby providing a practical protocol to assess the quality of individual lots. MDPI 2023-03-15 /pmc/articles/PMC10051840/ /pubmed/36983293 http://dx.doi.org/10.3390/jcm12062293 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Matsui, Kenji
Kinoshita, Yoshitaka
Inage, Yuka
Matsumoto, Naoto
Morimoto, Keita
Saito, Yatsumu
Takamura, Tsuyoshi
Matsunari, Hitomi
Yamanaka, Shuichiro
Nagashima, Hiroshi
Kobayashi, Eiji
Yokoo, Takashi
Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title_full Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title_fullStr Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title_full_unstemmed Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title_short Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
title_sort cryopreservation of fetal porcine kidneys for xenogeneic regenerative medicine
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051840/
https://www.ncbi.nlm.nih.gov/pubmed/36983293
http://dx.doi.org/10.3390/jcm12062293
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