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Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations

Bothrops atrox envenomations are common in the Brazilian Amazon. The venom of B. atrox is highly inflammatory, which results in severe local complications, including the formation of blisters. Moreover, there is little information on the immune mechanisms associated with this condition. Thus, a long...

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Autores principales: Coelho, Kerolaine Fonseca, Neves, Juliana Costa Ferreira, Ibiapina, Hiochelson Najibe Santos, Magalhães-Gama, Fábio, Barbosa, Fabiane Bianca Albuquerque, Silva, Flavio Souza, Wellmann, Irmgardt Alicia María, Sachett, Jacqueline Almeida Gonçalves, Tarragô, Andréa Monteiro, Ferreira, Luiz Carlos Lima, Malheiro, Adriana, Monteiro, Wuelton Marcelo, Costa, Allyson Guimarães
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051854/
https://www.ncbi.nlm.nih.gov/pubmed/36977086
http://dx.doi.org/10.3390/toxins15030196
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author Coelho, Kerolaine Fonseca
Neves, Juliana Costa Ferreira
Ibiapina, Hiochelson Najibe Santos
Magalhães-Gama, Fábio
Barbosa, Fabiane Bianca Albuquerque
Silva, Flavio Souza
Wellmann, Irmgardt Alicia María
Sachett, Jacqueline Almeida Gonçalves
Tarragô, Andréa Monteiro
Ferreira, Luiz Carlos Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
author_facet Coelho, Kerolaine Fonseca
Neves, Juliana Costa Ferreira
Ibiapina, Hiochelson Najibe Santos
Magalhães-Gama, Fábio
Barbosa, Fabiane Bianca Albuquerque
Silva, Flavio Souza
Wellmann, Irmgardt Alicia María
Sachett, Jacqueline Almeida Gonçalves
Tarragô, Andréa Monteiro
Ferreira, Luiz Carlos Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
author_sort Coelho, Kerolaine Fonseca
collection PubMed
description Bothrops atrox envenomations are common in the Brazilian Amazon. The venom of B. atrox is highly inflammatory, which results in severe local complications, including the formation of blisters. Moreover, there is little information on the immune mechanisms associated with this condition. Thus, a longitudinal study was carried out to characterize the profile of the cell populations and soluble immunological mediators in the peripheral blood and blisters in B. atrox patients s according to their clinical manifestations (mild and severe). A similar response in both B. atrox patient groups (MILD and SEV) was observed, with an increase in inflammatory monocytes, NKT, and T and B cells, as well as CCL2, CCL5, CXCL9, CXCL10, IL-1β and IL-10, when compared with the group of healthy blood donors. After the administration of antivenom, the participation of patrolling monocytes and IL-10 in the MILD group was observed. In the SEV group, the participation of B cells was observed, with high levels of CCL2 and IL-6. In the blister exudate, a hyperinflammatory profile was observed. In conclusion, we revealed the involvement of cell populations and soluble mediators in the immune response to B. atrox envenomation at the local and peripheral level, which is related to the onset and extent of the inflammation/clinical manifestation.
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spelling pubmed-100518542023-03-30 Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations Coelho, Kerolaine Fonseca Neves, Juliana Costa Ferreira Ibiapina, Hiochelson Najibe Santos Magalhães-Gama, Fábio Barbosa, Fabiane Bianca Albuquerque Silva, Flavio Souza Wellmann, Irmgardt Alicia María Sachett, Jacqueline Almeida Gonçalves Tarragô, Andréa Monteiro Ferreira, Luiz Carlos Lima Malheiro, Adriana Monteiro, Wuelton Marcelo Costa, Allyson Guimarães Toxins (Basel) Article Bothrops atrox envenomations are common in the Brazilian Amazon. The venom of B. atrox is highly inflammatory, which results in severe local complications, including the formation of blisters. Moreover, there is little information on the immune mechanisms associated with this condition. Thus, a longitudinal study was carried out to characterize the profile of the cell populations and soluble immunological mediators in the peripheral blood and blisters in B. atrox patients s according to their clinical manifestations (mild and severe). A similar response in both B. atrox patient groups (MILD and SEV) was observed, with an increase in inflammatory monocytes, NKT, and T and B cells, as well as CCL2, CCL5, CXCL9, CXCL10, IL-1β and IL-10, when compared with the group of healthy blood donors. After the administration of antivenom, the participation of patrolling monocytes and IL-10 in the MILD group was observed. In the SEV group, the participation of B cells was observed, with high levels of CCL2 and IL-6. In the blister exudate, a hyperinflammatory profile was observed. In conclusion, we revealed the involvement of cell populations and soluble mediators in the immune response to B. atrox envenomation at the local and peripheral level, which is related to the onset and extent of the inflammation/clinical manifestation. MDPI 2023-03-04 /pmc/articles/PMC10051854/ /pubmed/36977086 http://dx.doi.org/10.3390/toxins15030196 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coelho, Kerolaine Fonseca
Neves, Juliana Costa Ferreira
Ibiapina, Hiochelson Najibe Santos
Magalhães-Gama, Fábio
Barbosa, Fabiane Bianca Albuquerque
Silva, Flavio Souza
Wellmann, Irmgardt Alicia María
Sachett, Jacqueline Almeida Gonçalves
Tarragô, Andréa Monteiro
Ferreira, Luiz Carlos Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title_full Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title_fullStr Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title_full_unstemmed Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title_short Exploring the Profile of Cell Populations and Soluble Immunological Mediators in Bothrops atrox Envenomations
title_sort exploring the profile of cell populations and soluble immunological mediators in bothrops atrox envenomations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051854/
https://www.ncbi.nlm.nih.gov/pubmed/36977086
http://dx.doi.org/10.3390/toxins15030196
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