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Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests
Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052002/ https://www.ncbi.nlm.nih.gov/pubmed/36992364 http://dx.doi.org/10.3390/v15030654 |
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author | Prudencio, Carlos Roberto Gomes da Costa, Vivaldo Rocha, Leticia Barboza da Costa, Hernan Hermes Monteiro Orts, Diego José Belato da Silva Santos, Felipe Rocha Rahal, Paula Lino, Nikolas Alexander Borsato da Conceição, Pâmela Jóyce Previdelli Bittar, Cintia Machado, Rafael Rahal Guaragna Durigon, Edison Luiz Araujo, João Pessoa Polatto, Juliana Moutinho da Silva, Miriam Aparecida de Oliveira, Joyce Araújo Mitsunari, Thais Pereira, Lennon Ramos Andreata-Santos, Robert de Souza Ferreira, Luís Carlos Luz, Daniela Piazza, Roxane Maria Fontes |
author_facet | Prudencio, Carlos Roberto Gomes da Costa, Vivaldo Rocha, Leticia Barboza da Costa, Hernan Hermes Monteiro Orts, Diego José Belato da Silva Santos, Felipe Rocha Rahal, Paula Lino, Nikolas Alexander Borsato da Conceição, Pâmela Jóyce Previdelli Bittar, Cintia Machado, Rafael Rahal Guaragna Durigon, Edison Luiz Araujo, João Pessoa Polatto, Juliana Moutinho da Silva, Miriam Aparecida de Oliveira, Joyce Araújo Mitsunari, Thais Pereira, Lennon Ramos Andreata-Santos, Robert de Souza Ferreira, Luís Carlos Luz, Daniela Piazza, Roxane Maria Fontes |
author_sort | Prudencio, Carlos Roberto |
collection | PubMed |
description | Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses. |
format | Online Article Text |
id | pubmed-10052002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100520022023-03-30 Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests Prudencio, Carlos Roberto Gomes da Costa, Vivaldo Rocha, Leticia Barboza da Costa, Hernan Hermes Monteiro Orts, Diego José Belato da Silva Santos, Felipe Rocha Rahal, Paula Lino, Nikolas Alexander Borsato da Conceição, Pâmela Jóyce Previdelli Bittar, Cintia Machado, Rafael Rahal Guaragna Durigon, Edison Luiz Araujo, João Pessoa Polatto, Juliana Moutinho da Silva, Miriam Aparecida de Oliveira, Joyce Araújo Mitsunari, Thais Pereira, Lennon Ramos Andreata-Santos, Robert de Souza Ferreira, Luís Carlos Luz, Daniela Piazza, Roxane Maria Fontes Viruses Article Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses. MDPI 2023-02-28 /pmc/articles/PMC10052002/ /pubmed/36992364 http://dx.doi.org/10.3390/v15030654 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Prudencio, Carlos Roberto Gomes da Costa, Vivaldo Rocha, Leticia Barboza da Costa, Hernan Hermes Monteiro Orts, Diego José Belato da Silva Santos, Felipe Rocha Rahal, Paula Lino, Nikolas Alexander Borsato da Conceição, Pâmela Jóyce Previdelli Bittar, Cintia Machado, Rafael Rahal Guaragna Durigon, Edison Luiz Araujo, João Pessoa Polatto, Juliana Moutinho da Silva, Miriam Aparecida de Oliveira, Joyce Araújo Mitsunari, Thais Pereira, Lennon Ramos Andreata-Santos, Robert de Souza Ferreira, Luís Carlos Luz, Daniela Piazza, Roxane Maria Fontes Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title_full | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title_fullStr | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title_full_unstemmed | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title_short | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
title_sort | identification of zika virus ns1-derived peptides with potential applications in serological tests |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052002/ https://www.ncbi.nlm.nih.gov/pubmed/36992364 http://dx.doi.org/10.3390/v15030654 |
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