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Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice

Industrially originated trans-fatty acids (I-tFAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-tFAs), such as trans-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-tFA or R-tFA on the gut microbiome...

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Autores principales: Mohammadi, Farzad, Green, Miranda, Tolsdorf, Emma, Greffard, Karine, Leclercq, Mickael, Bilodeau, Jean-François, Droit, Arnaud, Foster, Jane, Bertrand, Nicolas, Rudkowska, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052023/
https://www.ncbi.nlm.nih.gov/pubmed/36986163
http://dx.doi.org/10.3390/nu15061433
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author Mohammadi, Farzad
Green, Miranda
Tolsdorf, Emma
Greffard, Karine
Leclercq, Mickael
Bilodeau, Jean-François
Droit, Arnaud
Foster, Jane
Bertrand, Nicolas
Rudkowska, Iwona
author_facet Mohammadi, Farzad
Green, Miranda
Tolsdorf, Emma
Greffard, Karine
Leclercq, Mickael
Bilodeau, Jean-François
Droit, Arnaud
Foster, Jane
Bertrand, Nicolas
Rudkowska, Iwona
author_sort Mohammadi, Farzad
collection PubMed
description Industrially originated trans-fatty acids (I-tFAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-tFAs), such as trans-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-tFA or R-tFA on the gut microbiome and fecal metabolite profile in mice after 7 and 28 days. Forty C57BL/6 mice were assigned to one of the four prepared formulations: lecithin nanovesicles, lecithin nanovesicles with EA or TPA, or water. Fecal samples and animals’ weights were collected on days 0, 7, and 28. Fecal samples were used to determine gut microbiome profiles by 16S rRNA sequencing and metabolite concentrations by GC/MS. At 28 days, TPA intake decreased the abundance of Staphylococcus sp55 but increased Staphylococcus sp119. EA intake also increased the abundance of Staphylococcus sp119 but decreased Ruminococcaceae UCG-014, Lachnospiraceae, and Clostridium sensu stricto 1 at 28 days. Fecal short-chain fatty acids were increased after TPA while decreased after EA after 7 and 28 days. This study shows that TPA and EA modify the abundance of specific microbial taxa and fecal metabolite profiles in distinct ways.
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spelling pubmed-100520232023-03-30 Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice Mohammadi, Farzad Green, Miranda Tolsdorf, Emma Greffard, Karine Leclercq, Mickael Bilodeau, Jean-François Droit, Arnaud Foster, Jane Bertrand, Nicolas Rudkowska, Iwona Nutrients Article Industrially originated trans-fatty acids (I-tFAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-tFAs), such as trans-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-tFA or R-tFA on the gut microbiome and fecal metabolite profile in mice after 7 and 28 days. Forty C57BL/6 mice were assigned to one of the four prepared formulations: lecithin nanovesicles, lecithin nanovesicles with EA or TPA, or water. Fecal samples and animals’ weights were collected on days 0, 7, and 28. Fecal samples were used to determine gut microbiome profiles by 16S rRNA sequencing and metabolite concentrations by GC/MS. At 28 days, TPA intake decreased the abundance of Staphylococcus sp55 but increased Staphylococcus sp119. EA intake also increased the abundance of Staphylococcus sp119 but decreased Ruminococcaceae UCG-014, Lachnospiraceae, and Clostridium sensu stricto 1 at 28 days. Fecal short-chain fatty acids were increased after TPA while decreased after EA after 7 and 28 days. This study shows that TPA and EA modify the abundance of specific microbial taxa and fecal metabolite profiles in distinct ways. MDPI 2023-03-16 /pmc/articles/PMC10052023/ /pubmed/36986163 http://dx.doi.org/10.3390/nu15061433 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mohammadi, Farzad
Green, Miranda
Tolsdorf, Emma
Greffard, Karine
Leclercq, Mickael
Bilodeau, Jean-François
Droit, Arnaud
Foster, Jane
Bertrand, Nicolas
Rudkowska, Iwona
Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title_full Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title_fullStr Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title_full_unstemmed Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title_short Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice
title_sort industrial and ruminant trans-fatty acids-enriched diets differentially modulate the microbiome and fecal metabolites in c57bl/6 mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052023/
https://www.ncbi.nlm.nih.gov/pubmed/36986163
http://dx.doi.org/10.3390/nu15061433
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