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Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium

Cobalt–chromium (Co-Cr)-based alloys are emerging with important characteristics for use in dentistry, but the knowledge of epigenetic mechanisms in endothelial cells has barely been achieved. In order to address this issue, we have prepared a previously Co-Cr-enriched medium to further treat endoth...

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Autores principales: da C. Fernandes, Célio Junior, da Silva, Rodrigo A. Foganholi, de Almeida, Gerson Santos, Ferreira, Marcel Rodrigues, de Morais, Paula Bertin, Bezerra, Fábio, Zambuzzi, Willian F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052026/
https://www.ncbi.nlm.nih.gov/pubmed/36976051
http://dx.doi.org/10.3390/jfb14030127
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author da C. Fernandes, Célio Junior
da Silva, Rodrigo A. Foganholi
de Almeida, Gerson Santos
Ferreira, Marcel Rodrigues
de Morais, Paula Bertin
Bezerra, Fábio
Zambuzzi, Willian F.
author_facet da C. Fernandes, Célio Junior
da Silva, Rodrigo A. Foganholi
de Almeida, Gerson Santos
Ferreira, Marcel Rodrigues
de Morais, Paula Bertin
Bezerra, Fábio
Zambuzzi, Willian F.
author_sort da C. Fernandes, Célio Junior
collection PubMed
description Cobalt–chromium (Co-Cr)-based alloys are emerging with important characteristics for use in dentistry, but the knowledge of epigenetic mechanisms in endothelial cells has barely been achieved. In order to address this issue, we have prepared a previously Co-Cr-enriched medium to further treat endothelial cells (HUVEC) for up to 72 h. Our data show there is important involvement with epigenetic machinery. Based on the data, it is believed that methylation balance in response to Co-Cr is finely modulated by DNMTs (DNA methyltransferases) and TETs (Tet methylcytosine dioxygenases), especially DNMT3B and both TET1 and TET2. Additionally, histone compaction HDAC6 (histone deacetylase 6) seems to develop a significant effect in endothelial cells. The requirement of SIRT1 seems to have a crucial role in this scenario. SIRT1 is associated with a capacity to modulate the expression of HIF-1α in response to hypoxia microenvironments, thus presenting a protective effect. As mentioned previously, cobalt is able to prevent HIF1A degradation and maintain hypoxia-related signaling in eukaryotic cells. Together, our results show, for the first time, a descriptive study reporting the relevance of epigenetic machinery in endothelial cells responding to cobalt–chromium, and it opens new perspectives to better understand their repercussions as prerequisites for driving cell adhesion, cell cycle progression, and angiogenesis surrounding this Co-Cr-based implantable device.
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spelling pubmed-100520262023-03-30 Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium da C. Fernandes, Célio Junior da Silva, Rodrigo A. Foganholi de Almeida, Gerson Santos Ferreira, Marcel Rodrigues de Morais, Paula Bertin Bezerra, Fábio Zambuzzi, Willian F. J Funct Biomater Article Cobalt–chromium (Co-Cr)-based alloys are emerging with important characteristics for use in dentistry, but the knowledge of epigenetic mechanisms in endothelial cells has barely been achieved. In order to address this issue, we have prepared a previously Co-Cr-enriched medium to further treat endothelial cells (HUVEC) for up to 72 h. Our data show there is important involvement with epigenetic machinery. Based on the data, it is believed that methylation balance in response to Co-Cr is finely modulated by DNMTs (DNA methyltransferases) and TETs (Tet methylcytosine dioxygenases), especially DNMT3B and both TET1 and TET2. Additionally, histone compaction HDAC6 (histone deacetylase 6) seems to develop a significant effect in endothelial cells. The requirement of SIRT1 seems to have a crucial role in this scenario. SIRT1 is associated with a capacity to modulate the expression of HIF-1α in response to hypoxia microenvironments, thus presenting a protective effect. As mentioned previously, cobalt is able to prevent HIF1A degradation and maintain hypoxia-related signaling in eukaryotic cells. Together, our results show, for the first time, a descriptive study reporting the relevance of epigenetic machinery in endothelial cells responding to cobalt–chromium, and it opens new perspectives to better understand their repercussions as prerequisites for driving cell adhesion, cell cycle progression, and angiogenesis surrounding this Co-Cr-based implantable device. MDPI 2023-02-26 /pmc/articles/PMC10052026/ /pubmed/36976051 http://dx.doi.org/10.3390/jfb14030127 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da C. Fernandes, Célio Junior
da Silva, Rodrigo A. Foganholi
de Almeida, Gerson Santos
Ferreira, Marcel Rodrigues
de Morais, Paula Bertin
Bezerra, Fábio
Zambuzzi, Willian F.
Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title_full Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title_fullStr Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title_full_unstemmed Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title_short Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt–Chromium
title_sort epigenetic differences arise in endothelial cells responding to cobalt–chromium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052026/
https://www.ncbi.nlm.nih.gov/pubmed/36976051
http://dx.doi.org/10.3390/jfb14030127
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