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Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli
The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052099/ https://www.ncbi.nlm.nih.gov/pubmed/36985169 http://dx.doi.org/10.3390/microorganisms11030595 |
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author | Mirzabekyan, Susanna Harutyunyan, Natalya Manvelyan, Anahit Malkhasyan, Lilit Balayan, Marine Miralimova, Shakhlo Chikindas, Michael L. Chistyakov, Vladimir Pepoyan, Astghik |
author_facet | Mirzabekyan, Susanna Harutyunyan, Natalya Manvelyan, Anahit Malkhasyan, Lilit Balayan, Marine Miralimova, Shakhlo Chikindas, Michael L. Chistyakov, Vladimir Pepoyan, Astghik |
author_sort | Mirzabekyan, Susanna |
collection | PubMed |
description | The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and E. coli might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts’ aquatic lifestyles. |
format | Online Article Text |
id | pubmed-10052099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100520992023-03-30 Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli Mirzabekyan, Susanna Harutyunyan, Natalya Manvelyan, Anahit Malkhasyan, Lilit Balayan, Marine Miralimova, Shakhlo Chikindas, Michael L. Chistyakov, Vladimir Pepoyan, Astghik Microorganisms Article The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and E. coli might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts’ aquatic lifestyles. MDPI 2023-02-27 /pmc/articles/PMC10052099/ /pubmed/36985169 http://dx.doi.org/10.3390/microorganisms11030595 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mirzabekyan, Susanna Harutyunyan, Natalya Manvelyan, Anahit Malkhasyan, Lilit Balayan, Marine Miralimova, Shakhlo Chikindas, Michael L. Chistyakov, Vladimir Pepoyan, Astghik Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title | Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title_full | Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title_fullStr | Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title_full_unstemmed | Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title_short | Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli |
title_sort | fish probiotics: cell surface properties of fish intestinal lactobacilli and escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052099/ https://www.ncbi.nlm.nih.gov/pubmed/36985169 http://dx.doi.org/10.3390/microorganisms11030595 |
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