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Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch

Microneedles are gaining a lot of attention in the context of sampling cutaneous biofluids such as capillary blood. Their minimal invasiveness and user-friendliness make them a prominent substitute for venous puncture or finger-pricking. Although the latter is suitable for self-sampling, the impract...

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Autores principales: Van Hileghem, Lorenz, Kushwaha, Shashwat, Piovesan, Agnese, Verboven, Pieter, Nicolaï, Bart, Reynaerts, Dominiek, Dal Dosso, Francesco, Lammertyn, Jeroen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052199/
https://www.ncbi.nlm.nih.gov/pubmed/36985022
http://dx.doi.org/10.3390/mi14030615
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author Van Hileghem, Lorenz
Kushwaha, Shashwat
Piovesan, Agnese
Verboven, Pieter
Nicolaï, Bart
Reynaerts, Dominiek
Dal Dosso, Francesco
Lammertyn, Jeroen
author_facet Van Hileghem, Lorenz
Kushwaha, Shashwat
Piovesan, Agnese
Verboven, Pieter
Nicolaï, Bart
Reynaerts, Dominiek
Dal Dosso, Francesco
Lammertyn, Jeroen
author_sort Van Hileghem, Lorenz
collection PubMed
description Microneedles are gaining a lot of attention in the context of sampling cutaneous biofluids such as capillary blood. Their minimal invasiveness and user-friendliness make them a prominent substitute for venous puncture or finger-pricking. Although the latter is suitable for self-sampling, the impracticality of manual handling and the difficulty of obtaining enough qualitative sample is driving the search for better solutions. In this context, hollow microneedle arrays (HMNAs) are particularly interesting for completely integrating sample-to-answer solutions as they create a duct between the skin and the sampling device. However, the fabrication of sharp-tipped HMNAs with a high aspect ratio (AR) is challenging, especially since a length of ≥1500 μm is desired to reach the blood capillaries. In this paper, we first described a novel two-step fabrication protocol for HMNAs in stainless steel by percussion laser drilling and subsequent micro-milling. The HMNAs were then integrated into a self-powered microfluidic sampling patch, containing a capillary pump which was optimized to generate negative pressure differences up to 40.9 ± 1.8 kPa. The sampling patch was validated in vitro, showing the feasibility of sampling 40 μL of liquid. It is anticipated that our proof-of-concept is a starting point for more sophisticated all-in-one biofluid sampling and point-of-care testing systems.
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spelling pubmed-100521992023-03-30 Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch Van Hileghem, Lorenz Kushwaha, Shashwat Piovesan, Agnese Verboven, Pieter Nicolaï, Bart Reynaerts, Dominiek Dal Dosso, Francesco Lammertyn, Jeroen Micromachines (Basel) Article Microneedles are gaining a lot of attention in the context of sampling cutaneous biofluids such as capillary blood. Their minimal invasiveness and user-friendliness make them a prominent substitute for venous puncture or finger-pricking. Although the latter is suitable for self-sampling, the impracticality of manual handling and the difficulty of obtaining enough qualitative sample is driving the search for better solutions. In this context, hollow microneedle arrays (HMNAs) are particularly interesting for completely integrating sample-to-answer solutions as they create a duct between the skin and the sampling device. However, the fabrication of sharp-tipped HMNAs with a high aspect ratio (AR) is challenging, especially since a length of ≥1500 μm is desired to reach the blood capillaries. In this paper, we first described a novel two-step fabrication protocol for HMNAs in stainless steel by percussion laser drilling and subsequent micro-milling. The HMNAs were then integrated into a self-powered microfluidic sampling patch, containing a capillary pump which was optimized to generate negative pressure differences up to 40.9 ± 1.8 kPa. The sampling patch was validated in vitro, showing the feasibility of sampling 40 μL of liquid. It is anticipated that our proof-of-concept is a starting point for more sophisticated all-in-one biofluid sampling and point-of-care testing systems. MDPI 2023-03-07 /pmc/articles/PMC10052199/ /pubmed/36985022 http://dx.doi.org/10.3390/mi14030615 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Van Hileghem, Lorenz
Kushwaha, Shashwat
Piovesan, Agnese
Verboven, Pieter
Nicolaï, Bart
Reynaerts, Dominiek
Dal Dosso, Francesco
Lammertyn, Jeroen
Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title_full Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title_fullStr Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title_full_unstemmed Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title_short Innovative Fabrication of Hollow Microneedle Arrays Enabling Blood Sampling with a Self-Powered Microfluidic Patch
title_sort innovative fabrication of hollow microneedle arrays enabling blood sampling with a self-powered microfluidic patch
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052199/
https://www.ncbi.nlm.nih.gov/pubmed/36985022
http://dx.doi.org/10.3390/mi14030615
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