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Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)

Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid β-citraurin is responsible for the peel color in “Newhall” orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on β-ci...

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Autores principales: Yue, Pengtao, Jiang, Zhenghua, Sun, Quan, Wei, Ranran, Yin, Yingzi, Xie, Zongzhou, Larkin, Robert M, Ye, Junli, Chai, Lijun, Deng, Xiuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052374/
https://www.ncbi.nlm.nih.gov/pubmed/36530163
http://dx.doi.org/10.1093/plcell/koac363
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author Yue, Pengtao
Jiang, Zhenghua
Sun, Quan
Wei, Ranran
Yin, Yingzi
Xie, Zongzhou
Larkin, Robert M
Ye, Junli
Chai, Lijun
Deng, Xiuxin
author_facet Yue, Pengtao
Jiang, Zhenghua
Sun, Quan
Wei, Ranran
Yin, Yingzi
Xie, Zongzhou
Larkin, Robert M
Ye, Junli
Chai, Lijun
Deng, Xiuxin
author_sort Yue, Pengtao
collection PubMed
description Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid β-citraurin is responsible for the peel color in “Newhall” orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on β-citraurin biosynthesis in citrus fruit remain unclear. Here, we determined that treatment with methyl jasmonate (MeJA) significantly promotes fruit coloration and β-citraurin production in “Newhall” orange. A MeJA treatment induced the expression of CsMYC2, which encodes a transcription factor that serves as a master regulator of jasmonate responses. CsMYC2 bound the promoter of the gene that encodes carotenoid cleavage dioxygenase 4b (CsCCD4b), the key gene for β-citraurin biosynthesis, and the promoters of genes that encode phytoene synthase (CsPSY), lycopene β-cyclase (CsLCYb), and β-carotene hydroxylase (CsBCH) and induced their expression. In addition, CsMYC2 promoted CsMPK6 expression. Notably, we found that CsMPK6 interacted with CsMYC2 and that this interaction decreased the stability and DNA-binding activity of CsMYC2. Thus, we conclude that negative feedback regulation attenuates JA signaling during the jasmonate-induced coloration of citrus fruit. Together, our findings indicate that jasmonates induce β-citraurin biosynthesis in citrus by activating a CsMPK6–CsMYC2 cascade, thereby affecting fruit coloration.
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spelling pubmed-100523742023-03-30 Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis) Yue, Pengtao Jiang, Zhenghua Sun, Quan Wei, Ranran Yin, Yingzi Xie, Zongzhou Larkin, Robert M Ye, Junli Chai, Lijun Deng, Xiuxin Plant Cell Research Article Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid β-citraurin is responsible for the peel color in “Newhall” orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on β-citraurin biosynthesis in citrus fruit remain unclear. Here, we determined that treatment with methyl jasmonate (MeJA) significantly promotes fruit coloration and β-citraurin production in “Newhall” orange. A MeJA treatment induced the expression of CsMYC2, which encodes a transcription factor that serves as a master regulator of jasmonate responses. CsMYC2 bound the promoter of the gene that encodes carotenoid cleavage dioxygenase 4b (CsCCD4b), the key gene for β-citraurin biosynthesis, and the promoters of genes that encode phytoene synthase (CsPSY), lycopene β-cyclase (CsLCYb), and β-carotene hydroxylase (CsBCH) and induced their expression. In addition, CsMYC2 promoted CsMPK6 expression. Notably, we found that CsMPK6 interacted with CsMYC2 and that this interaction decreased the stability and DNA-binding activity of CsMYC2. Thus, we conclude that negative feedback regulation attenuates JA signaling during the jasmonate-induced coloration of citrus fruit. Together, our findings indicate that jasmonates induce β-citraurin biosynthesis in citrus by activating a CsMPK6–CsMYC2 cascade, thereby affecting fruit coloration. Oxford University Press 2022-12-19 /pmc/articles/PMC10052374/ /pubmed/36530163 http://dx.doi.org/10.1093/plcell/koac363 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yue, Pengtao
Jiang, Zhenghua
Sun, Quan
Wei, Ranran
Yin, Yingzi
Xie, Zongzhou
Larkin, Robert M
Ye, Junli
Chai, Lijun
Deng, Xiuxin
Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title_full Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title_fullStr Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title_full_unstemmed Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title_short Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)
title_sort jasmonate activates a csmpk6-csmyc2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (citrus sinensis)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052374/
https://www.ncbi.nlm.nih.gov/pubmed/36530163
http://dx.doi.org/10.1093/plcell/koac363
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