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Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages

Understanding the normal variation of the sugarcane rhizosphere fungal community throughout its life cycle is essential for the development of agricultural practices for fungal and ecological health associated with the microbiota. Therefore, we performed high-throughput sequencing of 18S rDNA of soi...

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Autores principales: Liu, Qiang, Pang, Ziqin, Liu, Yueming, Fallah, Nyumah, Hu, Chaohua, Lin, Wenxiong, Yuan, Zhaonian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052501/
https://www.ncbi.nlm.nih.gov/pubmed/36982779
http://dx.doi.org/10.3390/ijms24065701
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author Liu, Qiang
Pang, Ziqin
Liu, Yueming
Fallah, Nyumah
Hu, Chaohua
Lin, Wenxiong
Yuan, Zhaonian
author_facet Liu, Qiang
Pang, Ziqin
Liu, Yueming
Fallah, Nyumah
Hu, Chaohua
Lin, Wenxiong
Yuan, Zhaonian
author_sort Liu, Qiang
collection PubMed
description Understanding the normal variation of the sugarcane rhizosphere fungal community throughout its life cycle is essential for the development of agricultural practices for fungal and ecological health associated with the microbiota. Therefore, we performed high-throughput sequencing of 18S rDNA of soil samples using the Illumina sequencing platform for correlation analysis of rhizosphere fungal community time series, covering information from 84 samples in four growth periods. The results revealed that the sugarcane rhizosphere fungi possessed the maximum fungal richness in Tillering. Rhizosphere fungi were closely associated with sugarcane growth, including Ascomycota, Basidiomycota, and Chytridiomycota, which showed high abundance in a stage-specific manner. Through the Manhattan plots, 10 fungal genera showed a decreasing trend throughout the sugarcane growth, and two fungal genera were significantly enriched at three stages of sugarcane growth (p < 0.05) including Pseudallescheria (Microascales, Microascaceae) and Nectriaceae (Hypocreales, Nectriaceae). In addition, soil pH, soil temperature, total nitrogen, and total potassium were critical drivers of fungal community structure at different stages of sugarcane growth. We also found that sugarcane disease status showed a significant and strong negative effect on selected soil properties by using structural equation modeling (SEM), suggesting that poor soil increases the likelihood of sugarcane disease. In addition, the assembly of sugarcane rhizosphere fungal community structure was mainly influenced by stochastic factors, but after the sugarcane root system became stable (Maturity), the stochastic contribution rate decreased to the lowest value. Our work provides a more extensive and solid basis for the biological control of sugarcane potential fungal diseases.
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spelling pubmed-100525012023-03-30 Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages Liu, Qiang Pang, Ziqin Liu, Yueming Fallah, Nyumah Hu, Chaohua Lin, Wenxiong Yuan, Zhaonian Int J Mol Sci Article Understanding the normal variation of the sugarcane rhizosphere fungal community throughout its life cycle is essential for the development of agricultural practices for fungal and ecological health associated with the microbiota. Therefore, we performed high-throughput sequencing of 18S rDNA of soil samples using the Illumina sequencing platform for correlation analysis of rhizosphere fungal community time series, covering information from 84 samples in four growth periods. The results revealed that the sugarcane rhizosphere fungi possessed the maximum fungal richness in Tillering. Rhizosphere fungi were closely associated with sugarcane growth, including Ascomycota, Basidiomycota, and Chytridiomycota, which showed high abundance in a stage-specific manner. Through the Manhattan plots, 10 fungal genera showed a decreasing trend throughout the sugarcane growth, and two fungal genera were significantly enriched at three stages of sugarcane growth (p < 0.05) including Pseudallescheria (Microascales, Microascaceae) and Nectriaceae (Hypocreales, Nectriaceae). In addition, soil pH, soil temperature, total nitrogen, and total potassium were critical drivers of fungal community structure at different stages of sugarcane growth. We also found that sugarcane disease status showed a significant and strong negative effect on selected soil properties by using structural equation modeling (SEM), suggesting that poor soil increases the likelihood of sugarcane disease. In addition, the assembly of sugarcane rhizosphere fungal community structure was mainly influenced by stochastic factors, but after the sugarcane root system became stable (Maturity), the stochastic contribution rate decreased to the lowest value. Our work provides a more extensive and solid basis for the biological control of sugarcane potential fungal diseases. MDPI 2023-03-16 /pmc/articles/PMC10052501/ /pubmed/36982779 http://dx.doi.org/10.3390/ijms24065701 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Qiang
Pang, Ziqin
Liu, Yueming
Fallah, Nyumah
Hu, Chaohua
Lin, Wenxiong
Yuan, Zhaonian
Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title_full Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title_fullStr Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title_full_unstemmed Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title_short Rhizosphere Fungal Dynamics in Sugarcane during Different Growth Stages
title_sort rhizosphere fungal dynamics in sugarcane during different growth stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052501/
https://www.ncbi.nlm.nih.gov/pubmed/36982779
http://dx.doi.org/10.3390/ijms24065701
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