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Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice

Nanoflowers (NFs) are flower-shaped nanoparticulate systems with a higher surface-to-volume ratio and good surface adsorption. Jaundice indicates yellow discoloration of skin, sclera, and mucus membrane and is a clinical indication of bilirubin accumulation in the blood which occurs as a consequence...

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Autores principales: Acharya, Kanchi, Prabhakar, Bala, Shende, Pravin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: YJBM 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052592/
https://www.ncbi.nlm.nih.gov/pubmed/37009196
http://dx.doi.org/10.59249/TJIV3644
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author Acharya, Kanchi
Prabhakar, Bala
Shende, Pravin
author_facet Acharya, Kanchi
Prabhakar, Bala
Shende, Pravin
author_sort Acharya, Kanchi
collection PubMed
description Nanoflowers (NFs) are flower-shaped nanoparticulate systems with a higher surface-to-volume ratio and good surface adsorption. Jaundice indicates yellow discoloration of skin, sclera, and mucus membrane and is a clinical indication of bilirubin accumulation in the blood which occurs as a consequence of the incapability of the liver to excrete bilirubin in the biliary tree or conjugate bilirubin and higher production of bilirubin in the body. Several methods have been developed so far for bilirubin estimation in jaundice like the spectrophotometric method, chemiluminescence method, etc., but biosensing methods provide advantages over traditional methods concerning the surface area, adsorption, particle size, and functional characteristics. The primary objective of the present research project was to formulate and examine the adsorbent nanoflowers-based biosensor for accurate, precise, and sensitive detection of bilirubin in jaundice. The particle size of adsorbent nanoflowers was found to be in the range of 300-600nm with the surface charge (zeta potential) in the range of -1.12 to -15.42 mV. Transmission electron microscopy and scanning electron microscopy images confirmed the flower-like morphological structure of adsorbent NFs. The adsorption efficiency of NFs for bilirubin adsorption was maximum at 94.13%. Comparative studies of bilirubin estimation in the pathological sample with adsorbent NFs and diagnostic kit displayed bilirubin concentration to be 1.0 mg/dL in adsorbent nanoflowers and 1.1 mg/dL with diagnostic kit indicating effective detection of bilirubin with adsorbent NFs. The nanoflower-based biosensor acts as a smart approach to elevate adsorption efficiency on the surface of nanoflower due to a higher surface-to-volume (SV) ratio.
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spelling pubmed-100525922023-03-31 Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice Acharya, Kanchi Prabhakar, Bala Shende, Pravin Yale J Biol Med Original Contribution Nanoflowers (NFs) are flower-shaped nanoparticulate systems with a higher surface-to-volume ratio and good surface adsorption. Jaundice indicates yellow discoloration of skin, sclera, and mucus membrane and is a clinical indication of bilirubin accumulation in the blood which occurs as a consequence of the incapability of the liver to excrete bilirubin in the biliary tree or conjugate bilirubin and higher production of bilirubin in the body. Several methods have been developed so far for bilirubin estimation in jaundice like the spectrophotometric method, chemiluminescence method, etc., but biosensing methods provide advantages over traditional methods concerning the surface area, adsorption, particle size, and functional characteristics. The primary objective of the present research project was to formulate and examine the adsorbent nanoflowers-based biosensor for accurate, precise, and sensitive detection of bilirubin in jaundice. The particle size of adsorbent nanoflowers was found to be in the range of 300-600nm with the surface charge (zeta potential) in the range of -1.12 to -15.42 mV. Transmission electron microscopy and scanning electron microscopy images confirmed the flower-like morphological structure of adsorbent NFs. The adsorption efficiency of NFs for bilirubin adsorption was maximum at 94.13%. Comparative studies of bilirubin estimation in the pathological sample with adsorbent NFs and diagnostic kit displayed bilirubin concentration to be 1.0 mg/dL in adsorbent nanoflowers and 1.1 mg/dL with diagnostic kit indicating effective detection of bilirubin with adsorbent NFs. The nanoflower-based biosensor acts as a smart approach to elevate adsorption efficiency on the surface of nanoflower due to a higher surface-to-volume (SV) ratio. YJBM 2023-03-31 /pmc/articles/PMC10052592/ /pubmed/37009196 http://dx.doi.org/10.59249/TJIV3644 Text en Copyright ©2023, Yale Journal of Biology and Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons CC BY-NC license, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use the material for commercial purposes.
spellingShingle Original Contribution
Acharya, Kanchi
Prabhakar, Bala
Shende, Pravin
Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title_full Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title_fullStr Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title_full_unstemmed Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title_short Design and Characterization of Nanoflowers-based Biosensor for Estimation of Bilirubin in Jaundice
title_sort design and characterization of nanoflowers-based biosensor for estimation of bilirubin in jaundice
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052592/
https://www.ncbi.nlm.nih.gov/pubmed/37009196
http://dx.doi.org/10.59249/TJIV3644
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