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Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease

Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to...

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Autores principales: Rodriguez, Daphne M., Hintze, Korry J., Rompato, Giovanni, Stewart, Eliza C., Barton, Abbey H., Mortensen-Curtis, Emily, Green, Porter A., Van Wettere, Arnaud J., Thomas, Aaron J., Benninghoff, Abby D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052649/
https://www.ncbi.nlm.nih.gov/pubmed/36986068
http://dx.doi.org/10.3390/nu15061338
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author Rodriguez, Daphne M.
Hintze, Korry J.
Rompato, Giovanni
Stewart, Eliza C.
Barton, Abbey H.
Mortensen-Curtis, Emily
Green, Porter A.
Van Wettere, Arnaud J.
Thomas, Aaron J.
Benninghoff, Abby D.
author_facet Rodriguez, Daphne M.
Hintze, Korry J.
Rompato, Giovanni
Stewart, Eliza C.
Barton, Abbey H.
Mortensen-Curtis, Emily
Green, Porter A.
Van Wettere, Arnaud J.
Thomas, Aaron J.
Benninghoff, Abby D.
author_sort Rodriguez, Daphne M.
collection PubMed
description Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from donor mice fed either the AIN basal diet or the TWD would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either the AIN diet or the TWD, using a 2 × 2 factorial experiment design. Time-matched FMT from the donor mice fed the TWD did not significantly enhance symptoms of colitis, colon epithelial inflammation, mucosal injury, or colon tumor burden in the recipient mice fed the AIN diet. Conversely, FMT from the AIN-fed donors did not impart a protective effect on the recipient mice fed the TWD. Likewise, the composition of fecal microbiomes of the recipient mice was also affected to a much greater extent by the diet they consumed than by the source of FMT. In summary, FMT from the donor mice fed either basal diet with differing colitis or tumor outcomes did not shift colitis symptoms or colon tumorigenesis in the recipient mice, regardless of the basal diet they consumed. These observations suggest that the gut microbiome may not contribute directly to the development of disease in this animal model.
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spelling pubmed-100526492023-03-30 Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease Rodriguez, Daphne M. Hintze, Korry J. Rompato, Giovanni Stewart, Eliza C. Barton, Abbey H. Mortensen-Curtis, Emily Green, Porter A. Van Wettere, Arnaud J. Thomas, Aaron J. Benninghoff, Abby D. Nutrients Article Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from donor mice fed either the AIN basal diet or the TWD would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either the AIN diet or the TWD, using a 2 × 2 factorial experiment design. Time-matched FMT from the donor mice fed the TWD did not significantly enhance symptoms of colitis, colon epithelial inflammation, mucosal injury, or colon tumor burden in the recipient mice fed the AIN diet. Conversely, FMT from the AIN-fed donors did not impart a protective effect on the recipient mice fed the TWD. Likewise, the composition of fecal microbiomes of the recipient mice was also affected to a much greater extent by the diet they consumed than by the source of FMT. In summary, FMT from the donor mice fed either basal diet with differing colitis or tumor outcomes did not shift colitis symptoms or colon tumorigenesis in the recipient mice, regardless of the basal diet they consumed. These observations suggest that the gut microbiome may not contribute directly to the development of disease in this animal model. MDPI 2023-03-09 /pmc/articles/PMC10052649/ /pubmed/36986068 http://dx.doi.org/10.3390/nu15061338 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodriguez, Daphne M.
Hintze, Korry J.
Rompato, Giovanni
Stewart, Eliza C.
Barton, Abbey H.
Mortensen-Curtis, Emily
Green, Porter A.
Van Wettere, Arnaud J.
Thomas, Aaron J.
Benninghoff, Abby D.
Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title_full Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title_fullStr Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title_full_unstemmed Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title_short Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease
title_sort basal diet fed to recipient mice was the driving factor for colitis and colon tumorigenesis, despite fecal microbiota transfer from mice with severe or mild disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052649/
https://www.ncbi.nlm.nih.gov/pubmed/36986068
http://dx.doi.org/10.3390/nu15061338
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