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Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers

The use of chemical fertilizers and pesticides has caused harmful impacts on the environment with the increase in economic burden. Biofertilizers are biological products containing living microorganisms capable of improving plant growth through eco-friendly mechanisms. In this work, three actinobact...

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Autores principales: Faddetta, Teresa, Polito, Giulia, Abbate, Loredana, Alibrandi, Pasquale, Zerbo, Marcello, Caldiero, Ciro, Reina, Chiara, Puccio, Guglielmo, Vaccaro, Edoardo, Abenavoli, Maria Rosa, Cavalieri, Vincenzo, Mercati, Francesco, Palumbo Piccionello, Antonio, Gallo, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052678/
https://www.ncbi.nlm.nih.gov/pubmed/36984814
http://dx.doi.org/10.3390/metabo13030374
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author Faddetta, Teresa
Polito, Giulia
Abbate, Loredana
Alibrandi, Pasquale
Zerbo, Marcello
Caldiero, Ciro
Reina, Chiara
Puccio, Guglielmo
Vaccaro, Edoardo
Abenavoli, Maria Rosa
Cavalieri, Vincenzo
Mercati, Francesco
Palumbo Piccionello, Antonio
Gallo, Giuseppe
author_facet Faddetta, Teresa
Polito, Giulia
Abbate, Loredana
Alibrandi, Pasquale
Zerbo, Marcello
Caldiero, Ciro
Reina, Chiara
Puccio, Guglielmo
Vaccaro, Edoardo
Abenavoli, Maria Rosa
Cavalieri, Vincenzo
Mercati, Francesco
Palumbo Piccionello, Antonio
Gallo, Giuseppe
author_sort Faddetta, Teresa
collection PubMed
description The use of chemical fertilizers and pesticides has caused harmful impacts on the environment with the increase in economic burden. Biofertilizers are biological products containing living microorganisms capable of improving plant growth through eco-friendly mechanisms. In this work, three actinobacterial strains Streptomyces violaceoruber, Streptomyces coelicolor, and Kocuria rhizophila were characterized for multiple plant growth promoting (PGP) traits such as indole acetic acid production, phosphate solubilization, N(2)-fixation, and drought and salt tolerance. Then, these strains were investigated for their secreted and cellular metabolome, revealing a rich arsenal of bioactive molecules, including antibiotics and siderophores, with S. violaceoruber being the most prolific strain. Furthermore, the in vivo assays, performed on tomato (Solanum lycopersicum L.), resulted in an improved germination index and the growth of seedlings from seeds treated with PGP actinobacteria, with a particular focus on S. violaceoruber cultures. In particular, this last strain, producing volatile organic compounds having antimicrobial activity, was able to modulate volatilome and exert control on the global DNA methylation of tomato seedlings. Thus, these results, confirming the efficacy of the selected actinobacteria strains in promoting plant growth and development by producing volatile and non-volatile bioactive molecules, can promote eco-friendly alternatives in sustainable agriculture.
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spelling pubmed-100526782023-03-30 Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers Faddetta, Teresa Polito, Giulia Abbate, Loredana Alibrandi, Pasquale Zerbo, Marcello Caldiero, Ciro Reina, Chiara Puccio, Guglielmo Vaccaro, Edoardo Abenavoli, Maria Rosa Cavalieri, Vincenzo Mercati, Francesco Palumbo Piccionello, Antonio Gallo, Giuseppe Metabolites Article The use of chemical fertilizers and pesticides has caused harmful impacts on the environment with the increase in economic burden. Biofertilizers are biological products containing living microorganisms capable of improving plant growth through eco-friendly mechanisms. In this work, three actinobacterial strains Streptomyces violaceoruber, Streptomyces coelicolor, and Kocuria rhizophila were characterized for multiple plant growth promoting (PGP) traits such as indole acetic acid production, phosphate solubilization, N(2)-fixation, and drought and salt tolerance. Then, these strains were investigated for their secreted and cellular metabolome, revealing a rich arsenal of bioactive molecules, including antibiotics and siderophores, with S. violaceoruber being the most prolific strain. Furthermore, the in vivo assays, performed on tomato (Solanum lycopersicum L.), resulted in an improved germination index and the growth of seedlings from seeds treated with PGP actinobacteria, with a particular focus on S. violaceoruber cultures. In particular, this last strain, producing volatile organic compounds having antimicrobial activity, was able to modulate volatilome and exert control on the global DNA methylation of tomato seedlings. Thus, these results, confirming the efficacy of the selected actinobacteria strains in promoting plant growth and development by producing volatile and non-volatile bioactive molecules, can promote eco-friendly alternatives in sustainable agriculture. MDPI 2023-03-02 /pmc/articles/PMC10052678/ /pubmed/36984814 http://dx.doi.org/10.3390/metabo13030374 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faddetta, Teresa
Polito, Giulia
Abbate, Loredana
Alibrandi, Pasquale
Zerbo, Marcello
Caldiero, Ciro
Reina, Chiara
Puccio, Guglielmo
Vaccaro, Edoardo
Abenavoli, Maria Rosa
Cavalieri, Vincenzo
Mercati, Francesco
Palumbo Piccionello, Antonio
Gallo, Giuseppe
Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title_full Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title_fullStr Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title_full_unstemmed Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title_short Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers
title_sort bioactive metabolite survey of actinobacteria showing plant growth promoting traits to develop novel biofertilizers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052678/
https://www.ncbi.nlm.nih.gov/pubmed/36984814
http://dx.doi.org/10.3390/metabo13030374
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