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PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype

Regulatory macrophages (Mreg) are a special cell type that present a potential therapeutic strategy for various inflammatory diseases. In vitro, Mreg generation mainly takes 7–10 days of treatment with chemicals, including cytokines. In the present study, we established a new approach for Mreg gener...

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Autores principales: Pham, Hoang Lan, Yang, Da Hyun, Chae, Woo Ri, Jung, Jong Hyeok, Hoang, Thi Xoan, Lee, Nae Yoon, Kim, Jae Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052727/
https://www.ncbi.nlm.nih.gov/pubmed/36985080
http://dx.doi.org/10.3390/mi14030673
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author Pham, Hoang Lan
Yang, Da Hyun
Chae, Woo Ri
Jung, Jong Hyeok
Hoang, Thi Xoan
Lee, Nae Yoon
Kim, Jae Young
author_facet Pham, Hoang Lan
Yang, Da Hyun
Chae, Woo Ri
Jung, Jong Hyeok
Hoang, Thi Xoan
Lee, Nae Yoon
Kim, Jae Young
author_sort Pham, Hoang Lan
collection PubMed
description Regulatory macrophages (Mreg) are a special cell type that present a potential therapeutic strategy for various inflammatory diseases. In vitro, Mreg generation mainly takes 7–10 days of treatment with chemicals, including cytokines. In the present study, we established a new approach for Mreg generation using a three-dimensional (3D) micropatterned polydimethylsiloxane (PDMS) surface coated with a natural biopolymer adhesive polydopamine (PDA) and the common cell adhesion peptide motif arginylglycylaspartic acid (RGD). The 3D PDMS surfaces were fabricated by photolithography and soft lithography techniques and were subsequently coated with an RGD+PDA mixture to form a surface that facilitates cell adhesion. Human monocytes (THP-1 cells) were cultured on different types of 2D or 3D micropatterns for four days, and the cell morphology, elongation, and Mreg marker expression were assessed using microscopic and flow cytometric analyses. The cells grown on the PDA+RGD-coated 3D micropatterns (20-µm width/20-µm space) exhibited the most elongated morphology and strongest expression levels of Mreg markers, such as CD163, CD206, CD209, CD274, MER-TK, TREM2, and DHRS9. The present study demonstrated that PDA+RGD-coated 3D PDMS micropatterns successfully induced Mreg-like cells from THP-1 cells within four days without the use of cytokines, suggesting a time- and cost-effective method to generate Mreg-like cells in vitro.
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spelling pubmed-100527272023-03-30 PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype Pham, Hoang Lan Yang, Da Hyun Chae, Woo Ri Jung, Jong Hyeok Hoang, Thi Xoan Lee, Nae Yoon Kim, Jae Young Micromachines (Basel) Article Regulatory macrophages (Mreg) are a special cell type that present a potential therapeutic strategy for various inflammatory diseases. In vitro, Mreg generation mainly takes 7–10 days of treatment with chemicals, including cytokines. In the present study, we established a new approach for Mreg generation using a three-dimensional (3D) micropatterned polydimethylsiloxane (PDMS) surface coated with a natural biopolymer adhesive polydopamine (PDA) and the common cell adhesion peptide motif arginylglycylaspartic acid (RGD). The 3D PDMS surfaces were fabricated by photolithography and soft lithography techniques and were subsequently coated with an RGD+PDA mixture to form a surface that facilitates cell adhesion. Human monocytes (THP-1 cells) were cultured on different types of 2D or 3D micropatterns for four days, and the cell morphology, elongation, and Mreg marker expression were assessed using microscopic and flow cytometric analyses. The cells grown on the PDA+RGD-coated 3D micropatterns (20-µm width/20-µm space) exhibited the most elongated morphology and strongest expression levels of Mreg markers, such as CD163, CD206, CD209, CD274, MER-TK, TREM2, and DHRS9. The present study demonstrated that PDA+RGD-coated 3D PDMS micropatterns successfully induced Mreg-like cells from THP-1 cells within four days without the use of cytokines, suggesting a time- and cost-effective method to generate Mreg-like cells in vitro. MDPI 2023-03-17 /pmc/articles/PMC10052727/ /pubmed/36985080 http://dx.doi.org/10.3390/mi14030673 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pham, Hoang Lan
Yang, Da Hyun
Chae, Woo Ri
Jung, Jong Hyeok
Hoang, Thi Xoan
Lee, Nae Yoon
Kim, Jae Young
PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title_full PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title_fullStr PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title_full_unstemmed PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title_short PDMS Micropatterns Coated with PDA and RGD Induce a Regulatory Macrophage-like Phenotype
title_sort pdms micropatterns coated with pda and rgd induce a regulatory macrophage-like phenotype
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10052727/
https://www.ncbi.nlm.nih.gov/pubmed/36985080
http://dx.doi.org/10.3390/mi14030673
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