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Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis
Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp met...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053014/ https://www.ncbi.nlm.nih.gov/pubmed/36984773 http://dx.doi.org/10.3390/metabo13030333 |
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author | Luo, Zhengzhong Yong, Kang Du, Zhenlong Huang, Yixin Zhou, Tao Ma, Li Yao, Xueping Shen, Liuhong Yu, Shumin Yan, Zuoting Cao, Suizhong |
author_facet | Luo, Zhengzhong Yong, Kang Du, Zhenlong Huang, Yixin Zhou, Tao Ma, Li Yao, Xueping Shen, Liuhong Yu, Shumin Yan, Zuoting Cao, Suizhong |
author_sort | Luo, Zhengzhong |
collection | PubMed |
description | Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp metabolism and inflammation in dairy cows with ketosis remains unclear. Therefore, blood samples from healthy (n = 10) and ketotic (n = 10) primiparous dairy cows were collected at the calving date and the day of ketosis diagnosis (7 days in milk (7 DIM)). Serum levels of non-esterified fatty acids (NEFA), BHBA, haptoglobin (HP), serum amyloid A (SAA), lipopolysaccharide, and cortisol were analyzed. Tryptophan and its metabolites were quantified using liquid chromatography–tandem mass spectrometry. At 7 DIM, the concentrations of NEFA, BHBA, HP, and SAA were higher and the levels of Trp, kynurenine (KYN), indoleacetic acid, indole-3-lactic acid, and 3-indoxyl sulfate were lower in the dairy cows with ketosis compared with those in the healthy cows. However, the KYN/Trp and melatonin/Trp ratios increased in the cows with ketosis. At the calving date, the serum lipopolysaccharide levels did not differ between the healthy and ketotic cows, whereas the levels of NEFA, HP, and cortisol increased in the ketotic cows. Correlation analysis showed that Trp deficiency and elevated Trp metabolism in the dairy cows occurred during ketosis. Overall, our results suggest that abnormal Trp metabolism may contribute to the pathogenesis of ketosis. |
format | Online Article Text |
id | pubmed-10053014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100530142023-03-30 Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis Luo, Zhengzhong Yong, Kang Du, Zhenlong Huang, Yixin Zhou, Tao Ma, Li Yao, Xueping Shen, Liuhong Yu, Shumin Yan, Zuoting Cao, Suizhong Metabolites Article Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp metabolism and inflammation in dairy cows with ketosis remains unclear. Therefore, blood samples from healthy (n = 10) and ketotic (n = 10) primiparous dairy cows were collected at the calving date and the day of ketosis diagnosis (7 days in milk (7 DIM)). Serum levels of non-esterified fatty acids (NEFA), BHBA, haptoglobin (HP), serum amyloid A (SAA), lipopolysaccharide, and cortisol were analyzed. Tryptophan and its metabolites were quantified using liquid chromatography–tandem mass spectrometry. At 7 DIM, the concentrations of NEFA, BHBA, HP, and SAA were higher and the levels of Trp, kynurenine (KYN), indoleacetic acid, indole-3-lactic acid, and 3-indoxyl sulfate were lower in the dairy cows with ketosis compared with those in the healthy cows. However, the KYN/Trp and melatonin/Trp ratios increased in the cows with ketosis. At the calving date, the serum lipopolysaccharide levels did not differ between the healthy and ketotic cows, whereas the levels of NEFA, HP, and cortisol increased in the ketotic cows. Correlation analysis showed that Trp deficiency and elevated Trp metabolism in the dairy cows occurred during ketosis. Overall, our results suggest that abnormal Trp metabolism may contribute to the pathogenesis of ketosis. MDPI 2023-02-23 /pmc/articles/PMC10053014/ /pubmed/36984773 http://dx.doi.org/10.3390/metabo13030333 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luo, Zhengzhong Yong, Kang Du, Zhenlong Huang, Yixin Zhou, Tao Ma, Li Yao, Xueping Shen, Liuhong Yu, Shumin Yan, Zuoting Cao, Suizhong Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title | Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title_full | Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title_fullStr | Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title_full_unstemmed | Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title_short | Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis |
title_sort | association between tryptophan metabolism and inflammatory biomarkers in dairy cows with ketosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053014/ https://www.ncbi.nlm.nih.gov/pubmed/36984773 http://dx.doi.org/10.3390/metabo13030333 |
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