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Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria
Genes encoding endolysins were identified and cloned from three different Escherichia coli bacteriophages, 10-24(13), PBEC30, and PBEC56. Putative antimicrobial peptide (AMP)-like C-terminal alpha helix structures with amphipathic natures were predicted from the three endolysins. Each gene was clone...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053066/ https://www.ncbi.nlm.nih.gov/pubmed/36992387 http://dx.doi.org/10.3390/v15030679 |
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author | Jeong, Tae-Hwan Hong, Hye-Won Kim, Min Soo Song, Miryoung Myung, Heejoon |
author_facet | Jeong, Tae-Hwan Hong, Hye-Won Kim, Min Soo Song, Miryoung Myung, Heejoon |
author_sort | Jeong, Tae-Hwan |
collection | PubMed |
description | Genes encoding endolysins were identified and cloned from three different Escherichia coli bacteriophages, 10-24(13), PBEC30, and PBEC56. Putative antimicrobial peptide (AMP)-like C-terminal alpha helix structures with amphipathic natures were predicted from the three endolysins. Each gene was cloned and expressed as hexahistidine-tagged forms, and the products were purified and characterized. The purified endolysins exhibited antibacterial activities against a variety of Gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumonia. Their antibacterial activities were improved by N-terminal fusion with an antimicrobial peptide, cecropin A. Minimum inhibitory concentrations (MIC) were as low as 4 μg/mL, depending on the targeted strain. The endolysins’ enzymatic activities were not affected by changes in pH at ranges from 5 to 10 and were stable at temperatures between 4 and 65 °C. The in vivo efficacies of the three endolysins were also demonstrated using Galleria melonella for infection models. |
format | Online Article Text |
id | pubmed-10053066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100530662023-03-30 Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria Jeong, Tae-Hwan Hong, Hye-Won Kim, Min Soo Song, Miryoung Myung, Heejoon Viruses Article Genes encoding endolysins were identified and cloned from three different Escherichia coli bacteriophages, 10-24(13), PBEC30, and PBEC56. Putative antimicrobial peptide (AMP)-like C-terminal alpha helix structures with amphipathic natures were predicted from the three endolysins. Each gene was cloned and expressed as hexahistidine-tagged forms, and the products were purified and characterized. The purified endolysins exhibited antibacterial activities against a variety of Gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumonia. Their antibacterial activities were improved by N-terminal fusion with an antimicrobial peptide, cecropin A. Minimum inhibitory concentrations (MIC) were as low as 4 μg/mL, depending on the targeted strain. The endolysins’ enzymatic activities were not affected by changes in pH at ranges from 5 to 10 and were stable at temperatures between 4 and 65 °C. The in vivo efficacies of the three endolysins were also demonstrated using Galleria melonella for infection models. MDPI 2023-03-04 /pmc/articles/PMC10053066/ /pubmed/36992387 http://dx.doi.org/10.3390/v15030679 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jeong, Tae-Hwan Hong, Hye-Won Kim, Min Soo Song, Miryoung Myung, Heejoon Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title | Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title_full | Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title_fullStr | Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title_full_unstemmed | Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title_short | Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria |
title_sort | characterization of three different endolysins effective against gram-negative bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053066/ https://www.ncbi.nlm.nih.gov/pubmed/36992387 http://dx.doi.org/10.3390/v15030679 |
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