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MicroRNAs as Biomarkers of Active Pulmonary TB Course

The spread of drug-resistant forms of TB dictates the need for surgical treatment in the complex of anti-tuberculosis measures in Russia. Most often, surgical intervention is performed in the case of pulmonary tuberculoma or fibrotic cavitary tuberculosis (FCT). This study is devoted to the search f...

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Autores principales: Shepelkova, Galina S., Evstifeev, Vladimir V., Tarasov, Ruslan V., Ergeshova, Anush E., Bagirov, Mamed A., Yeremeev, Vladimir V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053298/
https://www.ncbi.nlm.nih.gov/pubmed/36985200
http://dx.doi.org/10.3390/microorganisms11030626
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author Shepelkova, Galina S.
Evstifeev, Vladimir V.
Tarasov, Ruslan V.
Ergeshova, Anush E.
Bagirov, Mamed A.
Yeremeev, Vladimir V.
author_facet Shepelkova, Galina S.
Evstifeev, Vladimir V.
Tarasov, Ruslan V.
Ergeshova, Anush E.
Bagirov, Mamed A.
Yeremeev, Vladimir V.
author_sort Shepelkova, Galina S.
collection PubMed
description The spread of drug-resistant forms of TB dictates the need for surgical treatment in the complex of anti-tuberculosis measures in Russia. Most often, surgical intervention is performed in the case of pulmonary tuberculoma or fibrotic cavitary tuberculosis (FCT). This study is devoted to the search for biomarkers that characterize the course of disease in surgical TB patients. It is assumed that such biomarkers will help the surgeon decide on the timing of the planned operation. A number of serum microRNAs, potential regulators of inflammation and fibrosis in TB, selected on the basis of PCR-Array analysis, were considered as biomarkers. Quantitative real time polymerase chain reaction and receiver operating curves (ROC) were used to verify Array data and to estimate the ability of microRNAs (miRNAs) to discriminate between healthy controls, tuberculoma patients, and FCT patients. The study showed that miR-155, miR-191 and miR-223 were differentially expressed in serum of tuberculoma with “decay” and tuberculoma without “decay” patients. Another combination (miR-26a, miR-191, miR-222 and miR-320) forms a set to differentiate between tuberculoma with “decay” and FCT. Patients with tuberculoma without “decay” diagnosis differ from those with FCT in serum expression of miR-26a, miR-155, miR-191, miR-222 and miR-223. Further investigations are required to evaluate these sets on a larger population so as to set cut-off values that could be applied in laboratory diagnosis.
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spelling pubmed-100532982023-03-30 MicroRNAs as Biomarkers of Active Pulmonary TB Course Shepelkova, Galina S. Evstifeev, Vladimir V. Tarasov, Ruslan V. Ergeshova, Anush E. Bagirov, Mamed A. Yeremeev, Vladimir V. Microorganisms Article The spread of drug-resistant forms of TB dictates the need for surgical treatment in the complex of anti-tuberculosis measures in Russia. Most often, surgical intervention is performed in the case of pulmonary tuberculoma or fibrotic cavitary tuberculosis (FCT). This study is devoted to the search for biomarkers that characterize the course of disease in surgical TB patients. It is assumed that such biomarkers will help the surgeon decide on the timing of the planned operation. A number of serum microRNAs, potential regulators of inflammation and fibrosis in TB, selected on the basis of PCR-Array analysis, were considered as biomarkers. Quantitative real time polymerase chain reaction and receiver operating curves (ROC) were used to verify Array data and to estimate the ability of microRNAs (miRNAs) to discriminate between healthy controls, tuberculoma patients, and FCT patients. The study showed that miR-155, miR-191 and miR-223 were differentially expressed in serum of tuberculoma with “decay” and tuberculoma without “decay” patients. Another combination (miR-26a, miR-191, miR-222 and miR-320) forms a set to differentiate between tuberculoma with “decay” and FCT. Patients with tuberculoma without “decay” diagnosis differ from those with FCT in serum expression of miR-26a, miR-155, miR-191, miR-222 and miR-223. Further investigations are required to evaluate these sets on a larger population so as to set cut-off values that could be applied in laboratory diagnosis. MDPI 2023-02-28 /pmc/articles/PMC10053298/ /pubmed/36985200 http://dx.doi.org/10.3390/microorganisms11030626 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shepelkova, Galina S.
Evstifeev, Vladimir V.
Tarasov, Ruslan V.
Ergeshova, Anush E.
Bagirov, Mamed A.
Yeremeev, Vladimir V.
MicroRNAs as Biomarkers of Active Pulmonary TB Course
title MicroRNAs as Biomarkers of Active Pulmonary TB Course
title_full MicroRNAs as Biomarkers of Active Pulmonary TB Course
title_fullStr MicroRNAs as Biomarkers of Active Pulmonary TB Course
title_full_unstemmed MicroRNAs as Biomarkers of Active Pulmonary TB Course
title_short MicroRNAs as Biomarkers of Active Pulmonary TB Course
title_sort micrornas as biomarkers of active pulmonary tb course
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053298/
https://www.ncbi.nlm.nih.gov/pubmed/36985200
http://dx.doi.org/10.3390/microorganisms11030626
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