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Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ

Currently, the therapeutic effect of clopidogrel differs considerably among individuals and is thought to be closely related to the genetic polymorphism of CYP2C19. The CYP2C19*2 gene can reduce the antiplatelet aggregation effect of clopidogrel, which increases the risk of major cardiovascular adve...

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Autores principales: Chen, Siling, Yu, Rongjun, Li, Ying, Wu, Jiangling, Qiu, Jingfu, Huang, Xinyi, Xue, Jianjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053321/
https://www.ncbi.nlm.nih.gov/pubmed/36984950
http://dx.doi.org/10.3390/mi14030541
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author Chen, Siling
Yu, Rongjun
Li, Ying
Wu, Jiangling
Qiu, Jingfu
Huang, Xinyi
Xue, Jianjiang
author_facet Chen, Siling
Yu, Rongjun
Li, Ying
Wu, Jiangling
Qiu, Jingfu
Huang, Xinyi
Xue, Jianjiang
author_sort Chen, Siling
collection PubMed
description Currently, the therapeutic effect of clopidogrel differs considerably among individuals and is thought to be closely related to the genetic polymorphism of CYP2C19. The CYP2C19*2 gene can reduce the antiplatelet aggregation effect of clopidogrel, which increases the risk of major cardiovascular adverse events in patients. In this research, we report a new type of biosensor for the highly sensitive detection of the CYP2C19*2 gene based on exonuclease III assisted electric signal amplification and the use of calixarene to enrich electrical signal substances. Specifically, under the best conditions, the logarithmic concentrations of the analytes have a good linear relationship with the peak current in the range of 0.01 fM to 100 pM and the detection limit is 13.49 aM. The results have also shown that this method has good selectivity, high sensitivity, and stability, etc., and will provide a very promising application for the detection of the CYP2C19*2 gene and other biological molecules by replacing corresponding nucleic acid sequences.
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spelling pubmed-100533212023-03-30 Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ Chen, Siling Yu, Rongjun Li, Ying Wu, Jiangling Qiu, Jingfu Huang, Xinyi Xue, Jianjiang Micromachines (Basel) Article Currently, the therapeutic effect of clopidogrel differs considerably among individuals and is thought to be closely related to the genetic polymorphism of CYP2C19. The CYP2C19*2 gene can reduce the antiplatelet aggregation effect of clopidogrel, which increases the risk of major cardiovascular adverse events in patients. In this research, we report a new type of biosensor for the highly sensitive detection of the CYP2C19*2 gene based on exonuclease III assisted electric signal amplification and the use of calixarene to enrich electrical signal substances. Specifically, under the best conditions, the logarithmic concentrations of the analytes have a good linear relationship with the peak current in the range of 0.01 fM to 100 pM and the detection limit is 13.49 aM. The results have also shown that this method has good selectivity, high sensitivity, and stability, etc., and will provide a very promising application for the detection of the CYP2C19*2 gene and other biological molecules by replacing corresponding nucleic acid sequences. MDPI 2023-02-25 /pmc/articles/PMC10053321/ /pubmed/36984950 http://dx.doi.org/10.3390/mi14030541 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Siling
Yu, Rongjun
Li, Ying
Wu, Jiangling
Qiu, Jingfu
Huang, Xinyi
Xue, Jianjiang
Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title_full Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title_fullStr Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title_full_unstemmed Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title_short Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ
title_sort electrochemical biosensor for detection of the cyp2c19*2 allele based on exonuclease ⅲ
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053321/
https://www.ncbi.nlm.nih.gov/pubmed/36984950
http://dx.doi.org/10.3390/mi14030541
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