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Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique

EGCG is a catechin known for its antioxidant and anti-inflammatory characteristics. Vitamin B12 is an essential vitamin found in animal-derived products, and its deficiency may cause serious health problems such as anemia. The effectiveness of both catechin and vitamin B12 depends on their stability...

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Autores principales: Couto, Ana F., Favretto, Maéna, Paquis, Raphael, Estevinho, Berta N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053329/
https://www.ncbi.nlm.nih.gov/pubmed/36985516
http://dx.doi.org/10.3390/molecules28062544
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author Couto, Ana F.
Favretto, Maéna
Paquis, Raphael
Estevinho, Berta N.
author_facet Couto, Ana F.
Favretto, Maéna
Paquis, Raphael
Estevinho, Berta N.
author_sort Couto, Ana F.
collection PubMed
description EGCG is a catechin known for its antioxidant and anti-inflammatory characteristics. Vitamin B12 is an essential vitamin found in animal-derived products, and its deficiency may cause serious health problems such as anemia. The effectiveness of both catechin and vitamin B12 depends on their stability and bioavailability, which can be lost during industrial processes due to degradation when exposed to external factors. A potential solution to this issue is the microencapsulation, which protects the compounds from external agents. The current study aims to microencapsulate EGCG and vitamin B12 in a polymer matrix of biological origin, zein. Microencapsulation was performed using an electrospinning technique, and different concentrations of zein (1–30% w/v) and active compound (0.5–5% w/w) were tested, resulting in the production of micro/nanoparticles, fibers, or the mixture of both. The microstructures were analyzed and characterized in terms of morphology, release profile and kinetics, and encapsulation efficiency. High encapsulation efficiencies were obtained, and the highest were found in the samples with 1% w/w of active substance and 30% w/v of zein. Controlled release studies were conducted in deionized water and in an ethanolic solution, and five kinetic models were applied to the release profiles. The results indicated that the Weibull model was the best fit for the majority of results.
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spelling pubmed-100533292023-03-30 Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique Couto, Ana F. Favretto, Maéna Paquis, Raphael Estevinho, Berta N. Molecules Article EGCG is a catechin known for its antioxidant and anti-inflammatory characteristics. Vitamin B12 is an essential vitamin found in animal-derived products, and its deficiency may cause serious health problems such as anemia. The effectiveness of both catechin and vitamin B12 depends on their stability and bioavailability, which can be lost during industrial processes due to degradation when exposed to external factors. A potential solution to this issue is the microencapsulation, which protects the compounds from external agents. The current study aims to microencapsulate EGCG and vitamin B12 in a polymer matrix of biological origin, zein. Microencapsulation was performed using an electrospinning technique, and different concentrations of zein (1–30% w/v) and active compound (0.5–5% w/w) were tested, resulting in the production of micro/nanoparticles, fibers, or the mixture of both. The microstructures were analyzed and characterized in terms of morphology, release profile and kinetics, and encapsulation efficiency. High encapsulation efficiencies were obtained, and the highest were found in the samples with 1% w/w of active substance and 30% w/v of zein. Controlled release studies were conducted in deionized water and in an ethanolic solution, and five kinetic models were applied to the release profiles. The results indicated that the Weibull model was the best fit for the majority of results. MDPI 2023-03-10 /pmc/articles/PMC10053329/ /pubmed/36985516 http://dx.doi.org/10.3390/molecules28062544 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Couto, Ana F.
Favretto, Maéna
Paquis, Raphael
Estevinho, Berta N.
Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title_full Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title_fullStr Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title_full_unstemmed Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title_short Co-Encapsulation of Epigallocatechin-3-Gallate and Vitamin B12 in Zein Microstructures by Electrospinning/Electrospraying Technique
title_sort co-encapsulation of epigallocatechin-3-gallate and vitamin b12 in zein microstructures by electrospinning/electrospraying technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053329/
https://www.ncbi.nlm.nih.gov/pubmed/36985516
http://dx.doi.org/10.3390/molecules28062544
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